|
ori
|
rep_origin
|
origin
|
—
|
—
|
14306
|
|
lac operator
|
protein_bind
|
binding_site
|
—
|
—
|
11262
|
|
AmpR promoter
|
promoter
|
promoter
|
bla
|
—
|
10473
|
|
AmpR
|
CDS
|
antibiotic_resistance
|
bla
|
beta-lactamase
|
9953
|
|
T7 promoter
|
promoter
|
promoter
|
—
|
—
|
9298
|
|
6xHis
|
CDS
|
tag
|
—
|
6xHis affinity tag
|
7418
|
|
f1 ori
|
rep_origin
|
origin
|
—
|
—
|
6304
|
|
lacI
|
CDS
|
regulator
|
lacI
|
lac repressor
|
6012
|
|
T7 terminator
|
terminator
|
terminator
|
—
|
—
|
5978
|
|
rop
|
CDS
|
coding
|
rop
|
Rop protein, which maintains plasm…
|
5121
|
|
lacI promoter
|
promoter
|
promoter
|
lacI
|
—
|
4858
|
|
lac promoter
|
promoter
|
promoter
|
—
|
—
|
4760
|
|
KanR
|
CDS
|
antibiotic_resistance
|
aph(3')-Ia
|
aminoglycoside phosphotransferase
|
4195
|
|
CAP binding site
|
protein_bind
|
binding_site
|
—
|
—
|
4091
|
|
TEV site
|
CDS
|
coding
|
—
|
tobacco etch virus (TEV) protease …
|
3233
|
|
rrnB T1 terminator
|
terminator
|
terminator
|
Escherichia coli rrnB
|
—
|
3029
|
|
thrombin site
|
CDS
|
coding
|
—
|
thrombin recognition and cleavage …
|
2847
|
|
T3 promoter
|
promoter
|
promoter
|
—
|
—
|
2168
|
|
tac promoter
|
promoter
|
promoter
|
—
|
—
|
1930
|
|
HRV 3C site
|
CDS
|
coding
|
—
|
recognition and cleavage site for …
|
1803
|
|
GST
|
CDS
|
tag
|
—
|
glutathione S-transferase from Sch…
|
1750
|
|
lambda t0 terminator
|
terminator
|
terminator
|
—
|
—
|
1613
|
|
T7Te terminator
|
terminator
|
terminator
|
—
|
—
|
1437
|
|
rrnB T2 terminator
|
terminator
|
terminator
|
—
|
—
|
1298
|
|
CmR
|
CDS
|
coding
|
cat
|
chloramphenicol acetyltransferase
|
1247
|
|
URA3
|
CDS
|
coding
|
S. cerevisiae URA3
|
orotidine-5'-phosphate decarboxyla…
|
1220
|
|
attB1
|
protein_bind
|
binding_site
|
mutant version of attB
|
—
|
1201
|
|
cat promoter
|
promoter
|
promoter
|
—
|
—
|
1180
|
|
lacIq promoter
|
promoter
|
promoter
|
lacI (mutant)
|
—
|
1179
|
|
tet promoter
|
promoter
|
promoter
|
—
|
—
|
1174
|
|
attB2
|
protein_bind
|
binding_site
|
mutant version of attB
|
—
|
1144
|
|
2u ori
|
rep_origin
|
origin
|
—
|
—
|
1134
|
|
ADH1 terminator
|
terminator
|
terminator
|
S. cerevisiae ADH1
|
—
|
1065
|
|
SV40 poly(A) signal
|
polyA_signal
|
polya_signal
|
—
|
—
|
974
|
|
fd terminator
|
terminator
|
terminator
|
—
|
—
|
931
|
|
lacZ-alpha
|
CDS
|
reporter
|
lacZ fragment
|
LacZ-alpha fragment of beta-galact…
|
924
|
|
CYC1 terminator
|
terminator
|
terminator
|
S. cerevisiae CYC1
|
—
|
837
|
|
SV40 NLS
|
CDS
|
coding
|
—
|
nuclear localization signal of SV4…
|
822
|
|
MBP
|
CDS
|
tag
|
malE (mutated)
|
maltose binding protein from E. co…
|
805
|
|
SP6 promoter
|
promoter
|
promoter
|
—
|
—
|
791
|
|
NeoR/KanR
|
CDS
|
antibiotic_resistance
|
aph(3')-II (or nptII)
|
aminoglycoside phosphotransferase …
|
752
|
|
p15A ori
|
rep_origin
|
origin
|
—
|
—
|
710
|
|
URA3 promoter
|
promoter
|
promoter
|
S. cerevisiae URA3
|
—
|
691
|
|
Factor Xa site
|
CDS
|
coding
|
—
|
Factor Xa recognition and cleavage…
|
687
|
|
UAS
|
protein_bind
|
binding_site
|
—
|
—
|
646
|
|
FLAG
|
CDS
|
tag
|
—
|
FLAG(R) epitope tag, followed by a…
|
643
|
|
TEF terminator
|
terminator
|
terminator
|
—
|
—
|
620
|
|
tonB terminator
|
terminator
|
terminator
|
—
|
—
|
616
|
|
TEF promoter
|
promoter
|
promoter
|
—
|
—
|
602
|
|
T7 tag (gene 10 leader)
|
CDS
|
tag
|
—
|
leader peptide from bacteriophage …
|
590
|