pAH243

121147 Schizosaccharomyces pombe Yeast Expression

Plasmid Information

Purpose
Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast
Depositor
Katsunori Tanaka
Article
Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.
Tags
Flag
Promoter
S.pombe rrk1 and nmt41
Available Since
Feb. 21, 2019
Availability
Available

Sequence Information

Length
14,994 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 6
Label Gene Product Position Strand
3xFLAG — three tandem FLAG(R) epitope tags, foll… 1146..1212 Fwd
DNA Sequence
GACTATAAGGACCACGACGGAGACTACAAGGATCATGATATTGATTACAAAGACGATGACGATAAG
Protein Sequence
DYKDHDGDYKDHDIDYKDDDDK
SV40 NLS — nuclear localization signal of SV40 (si… 1218..1239 Fwd
DNA Sequence
CCAAAGAAGAAGCGGAAGGTC
Protein Sequence
PKKKRKV
Cas9 — Cas9 (Csn1) endonuclease from the Strep… 1263..5364 Fwd
DNA Sequence
GACAAGAAGTACAGCATCGGCCTGGACATCGGCACCAACTCTGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAATTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGAGCCCTGCTGTTCGACAGCGGCGAAACAGCCGAGGCCACCCGGCTGAAGAGAACCGCCAGAAGAAGATACACCAGACGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTGGTGGAAGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACCGACAAGGCCGACCTGCGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATCGCCCAGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCTGATTGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTGAGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATCCTGAGAGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCTCTATGATCAAGAGATACGACGAGCACCACCAGGACCTGACCCTGCTGAAAGCTCTCGTGCGGCAGCAGCTGCCTGAGAAGTACAAAGAGATTTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATTGACGGCGGAGCCAGCCAGGAAGAGTTCTACAAGTTCATCAAGCCCATCCTGGAAAAGATGGACGGCACCGAGGAACTGCTCGTGAAGCTGAACAGAGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGGGAGAGCTGCACGCCATTCTGCGGCGGCAGGAAGATTTTTACCCATTCCTGAAGGACAACCGGGAAAAGATCGAGAAGATCCTGACCTTCCGCATCCCCTACTACGTGGGCCCTCTGGCCAGGGGAAACAGCAGATTCGCCTGGATGACCAGAAAGAGCGAGGAAACCATCACCCCCTGGAACTTCGAGGAAGTGGTGGACAAGGGCGCTTCCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCGATAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCACCGTGTATAACGAGCTGACCAAAGTGAAATACGTGACCGAGGGAATGAGAAAGCCCGCCTTCCTGAGCGGCGAGCAGAAAAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAAGTGACCGTGAAGCAGCTGAAAGAGGACTACTTCAAGAAAATCGAGTGCTTCGACTCCGTGGAAATCTCCGGCGTGGAAGATCGGTTCAACGCCTCCCTGGGCACATACCACGATCTGCTGAAAATTATCAAGGACAAGGACTTCCTGGACAATGAGGAAAACGAGGACATTCTGGAAGATATCGTGCTGACCCTGACACTGTTTGAGGACAGAGAGATGATCGAGGAACGGCTGAAAACCTATGCCCACCTGTTCGACGACAAAGTGATGAAGCAGCTGAAGCGGCGGAGATACACCGGCTGGGGCAGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGTCCGGCAAGACAATCCTGGATTTCCTGAAGTCCGACGGCTTCGCCAACAGAAACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTTAAAGAGGACATCCAGAAAGCCCAGGTGTCCGGCCAGGGCGATAGCCTGCACGAGCACATTGCCAATCTGGCCGGCAGCCCCGCCATTAAGAAGGGCATCCTGCAGACAGTGAAGGTGGTGGACGAGCTCGTGAAAGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAAATGGCCAGAGAGAACCAGACCACCCAGAAGGGACAGAAGAACAGCCGCGAGAGAATGAAGCGGATCGAAGAGGGCATCAAAGAGCTGGGCAGCCAGATCCTGAAAGAACACCCCGTGGAAAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAATGGGCGGGATATGTACGTGGACCAGGAACTGGACATCAACCGGCTGTCCGACTACGATGTGGACCATATCGTGCCTCAGAGCTTTCTGAAGGACGACTCCATCGACAACAAGGTGCTGACCAGAAGCGACAAGAACCGGGGCAAGAGCGACAACGTGCCCTCCGAAGAGGTCGTGAAGAAGATGAAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATTACCCAGAGAAAGTTCGACAATCTGACCAAGGCCGAGAGAGGCGGCCTGAGCGAACTGGATAAGGCCGGCTTCATCAAGAGACAGCTGGTGGAAACCCGGCAGATCACAAAGCACGTGGCACAGATCCTGGACTCCCGGATGAACACTAAGTACGACGAGAATGACAAGCTGATCCGGGAAGTGAAAGTGATCACCCTGAAGTCCAAGCTGGTGTCCGATTTCCGGAAGGATTTCCAGTTTTACAAAGTGCGCGAGATCAACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTCGTGGGAACCGCCCTGATCAAAAAGTACCCTAAGCTGGAAAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAAATCGGCAAGGCTACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGCGGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATTTTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGGTGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGATAAGCTGATCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCGACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATAAGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGATCGACCTGTCTCAGCTGGGAGGCGAC
Protein Sequence
DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
nucleoplasmin NLS — bipartite nuclear localization signal f… 5364..5412 Fwd
DNA Sequence
AAAAGGCCGGCGGCCACGAAAAAGGCCGGCCAGGCAAAAAAGAAAAAG
Protein Sequence
KRPAATKKAGQAKKKK
AmpR bla beta-lactamase 11139..12000 Fwd
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
S. pombe ura4 Schizosaccharomyces pombe ura4 orotidine 5'-phosphate decarboxylase, r… 13609..14404 Rev
DNA Sequence
ATGGATGCTAGAGTATTTCAAAGCTATTCAGCTAGAGCTGAGGGGATGAAAAATCCCATTGCCAAGGAATTGTTGGCTTTGATGGAAGAAAAGCAAAGCAACTTGTCAGTCGCGGTCGATTTGACGAAGAAATCCGAAATCTTAGAATTGGTAGATAAAATTGGACCCTATGTCTGTGTTATCAAGACACATATTGACGTTGTCGAGGATTTCGACCAGGATATGGTAGAAAAACTGGTGGCCTTAGGTAAAAAGCATCGTTTTCTTATCTTTGAGGATCGCAAATTCGCAGACATTGGAAATACCGTCAAGCTACAATATGCATCTGGTGTGTACAAAATTGCTTCTTGGGCTCATATCACAAATTGCCATACAGTGCCAGGCGAGGGTATTATACAAGGCCTCAAAGAAGTTGGTTTACCTTTGGGACGTGGTCTCTTGCTTTTGGCTGAAATGTCATCCAAAGGCTCTTTGGCTACTGGTTCCTACACAGAGAAAACCTTAGAATGGTTTGAGAAGCATACCGATTTTTGCTTTGGCTTTATAGCTGGTCGTCGATTTCCTAACCTTCAAAGCGACTACATAACTATGTCCCCTGGTATCGGCTTGGATGTTAAAGGAGACGGGCTGGGACAGCAATATCGTACTCCTGAAGAAGTGATTGTAAACTGCGGTAGCGATATCATCATTGTTGGTCGTGGAGTCTATGGAGCTGGTCGTAATCCTGTTGTCGAAGCCAAGAGATATAGAGAAGCTGGTTGGAAGGCATATCAGCAAAGACTTTCTCAGCATTAA
Protein Sequence
MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKSEILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVKLQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSYTEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIVNCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH
promoter 4
Label Gene Product Position Strand
nmt1 P41 promoter — — 0..14994 Fwd
DNA Sequence
TCGCCATAAAAGACAGAATAAGTCATCAGCGGTTGTTTCATTTCCTATATTTTTTTTTTATTTTTTTATTTTTTAATAAGGGAAAATTTAACGTCTAAGGATACAGAAGATTGTTAGCACATTAAAGTAATAAAGGCTTAAGTAGTAAGTGCCTTAGCATGTTATTGTATTTCAAAGGACATAATCTAAAATAATAACAATATCATTTCTCACAAGTTATTCAATTTTCTTTTTTTTTTCTAATAATATCAAGAATGTATTATTTGTTTGACATAAGTCAACTAATTTATTTAATATGCTGGATTAATCTTGCAGACATGTAAATTAACAAGTTTTAGTCAAATAACGTTGAAGTTTCAATGAACTCAAATAATTTCTCTTTTTTTTTATATAACCATAATCTGATTTATATTTTCCGCAGGGATCAACTGAAGTTATGACATTTGGATTGGATCACTTATAACCTTGGTCGCCAAATAATACAAAAATCAGCGTTATAAAACAAAGAAGGTTTTTGTTAAGAAATTAATCCTCTTTCTTGATAAGAAAGTTGAACCGAAATTGCAGATACTGATATATGAAAATAATACCCACAATTTTGGGAATAGCGCAAGCCTCAATTTAAACAATAGGTGAGGACACATGATAATGACCTCAATGATTGTTAGAAGAAAAGAGCCTCATTACAAAATCGAAAAATGAATGGTTGGGTACAAGTTTCCAAAACATGGTAAAGTGGACTTTGCGTATGAGACGTAAATAGAAAAAAACACTTGTTATATGTTTTCTAGAATTATTGTTGTCTCTTTATGGTTGGATGATGCAAAATAGTAATTTCGGTTAGTTGCTGTAAAACACCACGAGACAAATAGATATGGATATTTATTAAATCAGGAAAAACGTAACTCTCGGCTACTGGATGGTTCAGTCACCCAACGATTACTGGGGAGAGAAAACAGGGCAAAAGCAAAGCTTAAAGGAATCCGATTGTCATTCGGCAATGTGCAGCGAAACTAAAAACCGGATAATGGACCTGTTAATCGAAACATTGAAGATAAAGGAAGAGGAATCCTGGCATATCATCAATTGAATAAGTTGAATTAATTATTTCAATCTCATTCTCACTTTCTGACTTATAGTCGCTTTGTTAAATC
rrk1 promoter/leader — — 8417..8774 Fwd
DNA Sequence
TTTGCTTATGTTGGTGGTAGTTGGCATGCGTAGACTGATGACTAGTCAGCAAGGAGCGTAGAACAGTCACACTCGTTATATATGTGCTTCCAAGAAAACTCAAGAATTTACCATTAGCAAACACTTTTTTGAAATGTTAGACATTTAAATGACGAAGGCATATAGAAGCTTTGAATAGGTGTTGTAAAGTGTTGATTTATGTGACGCTGAGGGTGCGCATGAAAGGAATGTTGGGTCACGATTATTAAACAGTTTGCTAGCTTGGACACTTGAGTATTGGAAGTTGTTGAATTCTAAAAAACTTTCAGTTGATTTGAATAGTTGCTGTTGCCAAAAAACATAACCTGTACCAAAGAA
AmpR promoter bla — 11034..11139 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
lac promoter — — 13082..13113 Fwd
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
CAP binding site — — 13046..13068 Fwd
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
lac operator — — 13120..13137 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
rep_origin 3
Label Gene Product Position Strand
ARS1 — — 6498..7280 Fwd
DNA Sequence
TAGGCATTTTGTTTAGTTAAAGGTACTTAAGTAATTAGTATAAACGAACCAATTTTATAATCAGGAAGTTAAGTGAATGGTAGCACATGTCGTAAAAATTGTGAATTTTTATTGAATAATATTTTAAATACAAGCCTTTCTAGACTAGGTATACTCATAAACATATATGAGCAAAAGGATAGAGGAGATTACATTGCATCTTCTACAAATTTATTTATTGCCCTTTACTGAAAAATTAAATAATGAGTACTAAATGATAAAAAGCGCTCAGTACAAGAAAGATTGCAAAAATATTGCATTCTTCATGAATTAAAGTTGCATATAAGGCATATTGAAAGTAATAGTACTAAAACAGCAGTTAGCGAAAATTAATAGAATTATATTCGCAAGACAATTGTGACATTAAATTAAAAAATTGTAGAATTTTTACTATCCTCTTTAACGCCATGAGCCTTTATAAAAAGGTTAAATTAGTTTTAACATTCTTTTTTTGAGTAAGAGTTTACAATTTATCAAAACCTGTGTTATTATATTCATTAGTTTCAATTTATTAGCATCTAGAGAAAAATCAATTGGCAGTTACATTGTTGGAATTTATGAAGAAAAAGAATCTACAACGGAGAATAAGTTGCTGATCTTTCCCTAAAATTGTATATTTTGCTGAGCTTATTTTGACATTTCGTTGAAGTTTTCTCTAATTCGCATTCATTTTAAGTAAAACAATGAGAAATAAAATTACAAAAAATACAAATTAAAATACAATTTTTAGCTATAATTATAGA
f1 ori — — 10297..10753 Fwd
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCTTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACTCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGTCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTT
ori — — 12170..12759 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA