| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| SV40 NLS | — | nuclear localization signal of SV40 (si… | 450..471 | Fwd | |
|
DNA Sequence
CCTAAGAAGAAGAGGAAGGTG
Protein Sequence
PKKKRKV
|
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| Myc | — | Myc (human c-Myc proto-oncogene) epitop… | 498..528 | Fwd | |
|
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
|
|||||
| Myc | — | Myc (human c-Myc proto-oncogene) epitop… | 537..567 | Fwd | |
|
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
|
|||||
| Myc | — | Myc (human c-Myc proto-oncogene) epitop… | 576..606 | Fwd | |
|
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
|
|||||
| Myc | — | Myc (human c-Myc proto-oncogene) epitop… | 615..645 | Fwd | |
|
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
|
|||||
| Myc | — | Myc (human c-Myc proto-oncogene) epitop… | 654..684 | Fwd | |
|
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
|
|||||
| Myc | — | Myc (human c-Myc proto-oncogene) epitop… | 717..747 | Fwd | |
|
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
|
|||||
| GFP11 | — | 11th beta-strand of superfolder GFP | 1410..1458 | Fwd | |
|
DNA Sequence
CGCGACCACATGGTGCTGCATGAGTATGTCAACGCTGCGGGAATTACC
Protein Sequence
RDHMVLHEYVNAAGIT
|
|||||
| AmpR | bla | beta-lactamase | 3153..4014 | Rev | |
|
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| SV40 poly(A) signal | — | — | 1605..1740 | Fwd | |
|
DNA Sequence
GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac promoter | — | — | 2040..2071 | Rev | |
|
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
|
|||||
| AmpR promoter | bla | — | 4014..4119 | Rev | |
|
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| attB4 | mutant version of attB | — | 52..73 | Fwd | |
|
DNA Sequence
CAACTTTGTATAGAAAAGTTG
|
|||||
| attB1 | mutant version of attB | — | 411..436 | Fwd | |
|
DNA Sequence
GCAAGTTTGTACAAAAAAGCAGGCT
|
|||||
| attB2 | mutant version of attB | — | 1513..1538 | Rev | |
|
DNA Sequence
CCCACTTTGTACAAGAAAGCTGGGT
|
|||||
| attB3 | mutant version of attB | — | 1563..1584 | Rev | |
|
DNA Sequence
CAACTATGTATAATAAAGTTG
|
|||||
| lac operator | — | — | 2016..2033 | Fwd | |
|
DNA Sequence
TTGTTATCCGCTCACAA
|
|||||
| CAP binding site | — | — | 2085..2107 | Fwd | |
|
DNA Sequence
ATGAGTGAGCTAACTCACATTA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| ori | — | — | 2394..2983 | Rev | |
|
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
|
|||||