pET30 SUMO-1 G97

13381 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Mary Dasso
Article
Saitoh et al Curr Biol. 1998 Jan 15. 8(2):121-4.
Insert
SUMO-1, G97 (SUMO1 Human)
Tags
His
Mutation
G97 mutant: lacks 4 amino acids from the C-termin…
Available Since
Nov. 16, 2006
Availability
Available

Sequence Information

Length
426 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 12..30 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 39..57 Fwd
DNA Sequence
CTGGTGCCACGCGGTTCT
Protein Sequence
LVPRGS
S-Tag — affinity and epitope tag derived from p… 63..108 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
enterokinase site — enterokinase recognition and cleavage s… 123..138 Fwd
DNA Sequence
GACGACGACGACAAG
Protein Sequence
DDDDK