pKM493

140191 Synthetic Bacterial Expression

Plasmid Information

Purpose
ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.
Depositor
Kenan Murphy
Article
Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-
Insert
TEV-Flag-Gly4-eGFP
Tags
Flag + eGFP
Promoter
PGroEL
Available Since
Jan. 2, 2024
Availability
Available

Sequence Information

Length
4,246 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
FLAG — FLAG(R) epitope tag, followed by an ent… 531..555 Fwd
DNA Sequence
GACTACAAGGACGACGACGACAAG
Protein Sequence
DYKDDDDK
EGFP — the original enhanced GFP (Yang et al.,… 567..1284 Fwd
DNA Sequence
ATGGTGTCGAAGGGCGAGGAGCTGTTCACCGGCGTCGTGCCCATCCTGGTCGAGCTGGACGGCGACGTGAACGGCCACAAGTTCAGCGTCTCGGGCGAGGGCGAGGGCGACGCGACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCGGTCCCCTGGCCGACCCTGGTGACCACCCTGACCTACGGCGTGCAGTGTTTCTCGCGGTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGAGCGCGATGCCGGAGGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGGGCCGAGGTCAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAAGACGGCAACATCCTGGGCCACAAGCTGGAGTACAACTACAACTCGCACAACGTCTACATCATGGCGGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTCCAGCTGGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTCCTGCTGCCGGACAACCACTACCTGAGCACCCAGTCGGCGCTGAGCAAGGACCCGAACGAGAAGCGGGACCACATGGTCCTGCTGGAGTTCGTGACCGCCGCGGGCATCACCCTGGGCATGGACGAGCTGTACAAG
Protein Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
protein_bind 1
Label Gene Product Position Strand
Bxb1 attB — — 467..505 Fwd
DNA Sequence
GGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGATCAT
rep_origin 1
Label Gene Product Position Strand
ori — — 1496..2085 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAGGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 2
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB — 0..4246 Rev
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTAT
rrnB T2 terminator — — 1302..1330 Fwd
DNA Sequence
AGAAGGCCATCCTGACGGATGGCCTTTT