pnYC-CvH-Hs4EHP_1-234_AE

148561 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial Expression of Hs4EHP_1-234
Depositor
Elisa Izaurralde
Article
Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.
Insert
Hs4EHP_1-234 (EIF4E2 Human)
Available Since
March 16, 2023
Availability
Available

Sequence Information

Length
5,120 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
TEV site — tobacco etch virus (TEV) protease recog… 3205..3226 Fwd
DNA Sequence
GAAAACCTTTACTTCCAGGGC
Protein Sequence
ENLYFQG
6xHis — 6xHis affinity tag 3253..3271 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 3
Label Gene Product Position Strand
T7 promoter — — 2400..2419 Fwd
DNA Sequence
TAATACGACTCACTATAGG
tet promoter — — 3587..3616 Rev
DNA Sequence
TTGACAGCTTATCATCGATAAGCTTTAAT
cat promoter — — 4798..4901 Fwd
DNA Sequence
TGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAA
protein_bind 1
Label Gene Product Position Strand
lac operator — — 2419..2444 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
rep_origin 1
Label Gene Product Position Strand
p15A ori — — 3727..4273 Fwd
DNA Sequence
TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
T7 terminator — — 3365..3413 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG