pGBW-m4046531

148995 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
Depositor
Ginkgo Bioworks, Benjie Chen
Insert
SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
Tags
Cleavable thrombin;6xHIS
Mutation
wt
Promoter
pTEF1
Available Since
May 14, 2020
Availability
Available

Sequence Information

Length
3,051 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
thrombin site — thrombin recognition and cleavage site 2865..2883 Fwd
DNA Sequence
CTCGTGCCGAGAGGTTCT
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 2883..2901 Fwd
DNA Sequence
CACCATCATCACCATCAT
Protein Sequence
HHHHHH