pET28(a)-hFX-HA

14991 Homo sapiens Bacterial Expression

Plasmid Information

Depositor

Michael Ristow

Article

Dhe-Paganon et al J Biol Chem. 2000 Oct 6. 275(40):30753-6

Insert

human frataxin cDNA (FXN Human)

Tags

AU1, HA, and HIS

Mutation

contains cDNA coding sequence for amino acids 88-…

Available Since

Aug. 17, 2007

Availability

Available

Sequence Information

Length

715 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

CDS 5

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	30..48	Rev
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH
HA	—	HA (human influenza hemagglutinin) epit…	108..135	Rev
DNA Sequence TACCCTTACGACGTACCAGACTACGCA Protein Sequence YPYDVPDYA
T7 tag (gene 10 leader)	—	leader peptide from bacteriophage T7 ge…	513..546	Rev
DNA Sequence ATGGCTAGCATGACTGGTGGACAGCAAATGGGT Protein Sequence MASMTGGQQMG
thrombin site	—	thrombin recognition and cleavage site	549..567	Rev
DNA Sequence CTGGTGCCGCGCGGCAGC Protein Sequence LVPRGS
6xHis	—	6xHis affinity tag	576..594	Rev
DNA Sequence CATCATCATCATCATCAC Protein Sequence HHHHHH

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	653..670	Fwd
DNA Sequence TTGTTATCCGCTCACAA