pET28(a)-hFX(I154F)-HA

14992 Homo sapiens Bacterial Expression

Plasmid Information

Depositor

Michael Ristow

Insert

human frataxin cDNA (FXN Human)

Tags

AU1, HA, and HIS

Mutation

contains cDNA coding sequence for amino acids 88-…

Available Since

Aug. 17, 2007

Availability

Available

Sequence Information

Length

881 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

CDS 5

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	35..53	Rev
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH
HA	—	HA (human influenza hemagglutinin) epit…	113..140	Rev
DNA Sequence TACCCTTACGACGTACCAGACTACGCA Protein Sequence YPYDVPDYA
T7 tag (gene 10 leader)	—	leader peptide from bacteriophage T7 ge…	518..551	Rev
DNA Sequence ATGGCTAGCATGACTGGTGGACAGCAAATGGGT Protein Sequence MASMTGGQQMG
thrombin site	—	thrombin recognition and cleavage site	554..572	Rev
DNA Sequence CTGGTGCCGCGCGGCAGC Protein Sequence LVPRGS
6xHis	—	6xHis affinity tag	581..599	Rev
DNA Sequence CATCATCATCATCATCAC Protein Sequence HHHHHH

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	679..698	Rev
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	654..679	Fwd
DNA Sequence GGAATTGTTATCCGCTCACAATTCC