pET41a-MED19

15430 Mus musculus Bacterial Expression

Plasmid Information

Depositor
Joan Conaway, Ronald Conaway
Article
Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-
Insert
MED19 (Med19 Mouse)
Tags
GST and His
Available Since
Aug. 10, 2007
Availability
Available

Sequence Information

Length
934 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 30..48 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 57..75 Fwd
DNA Sequence
CTGGTGCCACGCGGTAGT
Protein Sequence
LVPRGS
S-Tag — affinity and epitope tag derived from p… 90..135 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
enterokinase site — enterokinase recognition and cleavage s… 165..180 Fwd
DNA Sequence
GATGACGACGACAAG
Protein Sequence
DDDDK