pET41a-MED18

15433 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Joan Conaway, Ronald Conaway
Article
Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-
Insert
MED18 (MED18 Human)
Tags
GST and His
Mutation
N/A
Available Since
Aug. 10, 2007
Availability
Available

Sequence Information

Length
682 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 27..45 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 54..72 Fwd
DNA Sequence
CTGGTGCCACGCGGTAGT
Protein Sequence
LVPRGS
S-Tag — affinity and epitope tag derived from p… 87..132 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
enterokinase site — enterokinase recognition and cleavage s… 162..177 Fwd
DNA Sequence
GATGACGACGACAAG
Protein Sequence
DDDDK