pST39-His/Vps27(345-440) - Hse(275-375)

17638 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
James Hurley
Article
Prag et al Dev Cell. 2007 Jun . 12(6):973-86.
Insert
His/Vps27(345-440) – Hse1(275-375) (VPS27, HSE1 Budding Yeast)
Available Since
March 28, 2008
Availability
Available

Sequence Information

Length
697 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 150..168 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 189..210 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG