pZac2.1 gfaABC1D-Aqp4-BioID2-BioID2-HA

Plasmid Information

Purpose

Expresses Aquaporin-4 fused with BioID2 at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Aqp4-BioID2-BioID2-HA

Sequence Information

Length

7,540 bp

Topology

circular

Molecule Type

other DNA

View on AddGene.org

Features

CDS 5

Label	Gene	Product	Position	Strand
BioID2	—	promiscuous R40G mutant of a biotin pro…	2370..3069	Fwd
DNA Sequence ATGTTCAAAAATCTTATTTGGCTGAAGGAAGTTGATAGCACCCAGGAGCGGCTTAAGGAATGGAATGTCTCCTATGGCACTGCACTCGTCGCCGATAGACAGACCAAAGGTAGAGGCGGTCTCGGACGGAAGTGGTTGAGTCAGGAGGGCGGTCTTTACTTCTCTTTCTTGTTGAATCCAAAAGAGTTTGAAAATTTGTTGCAACTTCCACTGGTCTTGGGACTCTCCGTGAGCGAGGCACTCGAAGAGATTACAGAGATACCATTTAGTCTGAAGTGGCCCAACGACGTTTATTTTCAGGAGAAAAAGGTTTCAGGGGTCCTTTGTGAGCTCAGTAAGGACAAGCTGATAGTGGGAATCGGTATAAACGTGAATCAAAGAGAGATCCCTGAAGAGATCAAGGATCGGGCCACAACACTTTATGAAATCACTGGAAAGGATTGGGACAGAAAGGAGGTCTTGCTGAAGGTTCTCAAGCGAATTTCCGAAAATCTCAAGAAATTTAAGGAAAAATCCTTTAAAGAGTTCAAAGGAAAAATTGAGTCAAAGATGTTGTACCTTGGAGAGGAGGTTAAACTCTTGGGAGAAGGCAAGATTACCGGGAAACTTGTGGGCCTGAGTGAAAAGGGCGGTGCTTTGATACTTACAGAGGAAGGCATAAAGGAGATACTGTCCGGCGAATTTAGCTTGCGACGGTCA Protein Sequence MFKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWLSQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVSGVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISENLKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEILSGEFSLRRS
BioID2	—	promiscuous R40G mutant of a biotin pro…	3153..3852	Fwd
DNA Sequence ATGTTCAAAAATCTTATTTGGCTGAAGGAAGTTGATAGCACCCAGGAGCGGCTTAAGGAATGGAATGTCTCCTATGGCACTGCACTCGTCGCCGATAGACAGACCAAAGGTAGAGGCGGTCTCGGACGGAAGTGGTTGAGTCAGGAGGGCGGTCTTTACTTCTCTTTCTTGTTGAATCCAAAAGAGTTTGAAAATTTGTTGCAACTTCCACTGGTCTTGGGACTCTCCGTGAGCGAGGCACTCGAAGAGATTACAGAGATACCATTTAGTCTGAAGTGGCCCAACGACGTTTATTTTCAGGAGAAAAAGGTTTCAGGGGTCCTTTGTGAGCTCAGTAAGGACAAGCTGATAGTGGGAATCGGTATAAACGTGAATCAAAGAGAGATCCCTGAAGAGATCAAGGATCGGGCCACAACACTTTATGAAATCACTGGAAAGGATTGGGACAGAAAGGAGGTCTTGCTGAAGGTTCTCAAGCGAATTTCCGAAAATCTCAAGAAATTTAAGGAAAAATCCTTTAAAGAGTTCAAAGGAAAAATTGAGTCAAAGATGTTGTACCTTGGAGAGGAGGTTAAACTCTTGGGAGAAGGCAAGATTACCGGGAAACTTGTGGGCCTGAGTGAAAAGGGCGGTGCTTTGATACTTACAGAGGAAGGCATAAAGGAGATACTGTCCGGCGAATTTAGCTTGCGACGGTCA Protein Sequence MFKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWLSQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVSGVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISENLKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEILSGEFSLRRS
HA	—	HA (human influenza hemagglutinin) epit…	3861..3888	Fwd
DNA Sequence TACCCTTACGATGTACCGGATTACGCA Protein Sequence YPYDVPDYA
Factor Xa site	—	Factor Xa recognition and cleavage site	4383..4395	Rev
DNA Sequence ATTGAGGGCCGA Protein Sequence IEGR
AmpR	bla	beta-lactamase	5739..6600	Fwd
DNA Sequence ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA Protein Sequence MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW

intron 1

Label	Gene	Product	Position	Strand
chimeric intron	—	—	1238..1371	Fwd
DNA Sequence GTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAG

misc_feature 1

Label	Gene	Product	Position	Strand
WPRE	—	—	3912..4501	Fwd
DNA Sequence AATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAATCATCGTCCTTTCCTTGGCTGCTCGCCTATGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGC

polyA_signal 1

Label	Gene	Product	Position	Strand
SV40 poly(A) signal	—	—	4564..4686	Fwd
DNA Sequence TAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT

promoter 3

Label	Gene	Product	Position	Strand
lac promoter	—	—	142..173	Fwd
DNA Sequence TTTACACTTTATGCTTCCGGCTCGTATGTTG
T7 promoter	—	—	1415..1434	Fwd
DNA Sequence TAATACGACTCACTATAGG
AmpR promoter	bla	—	5634..5739	Fwd
DNA Sequence CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT

protein_bind 2

Label	Gene	Product	Position	Strand
CAP binding site	—	—	106..128	Fwd
DNA Sequence TAATGTGAGTTAGCTCACTCAT
lac operator	—	—	180..197	Fwd
DNA Sequence TTGTGAGCGGATAACAA

rep_origin 2

Label	Gene	Product	Position	Strand
f1 ori	—	—	5153..5609	Fwd
DNA Sequence ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTT
ori	—	—	6770..7359	Fwd
DNA Sequence TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA