| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| BioID2 | — | promiscuous R40G mutant of a biotin pro… | 2617..3316 | Fwd | |
|
DNA Sequence
ATGTTCAAAAATCTTATTTGGCTGAAGGAAGTTGATAGCACCCAGGAGCGGCTTAAGGAATGGAATGTCTCCTATGGCACTGCACTCGTCGCCGATAGACAGACCAAAGGTAGAGGCGGTCTCGGACGGAAGTGGTTGAGTCAGGAGGGCGGTCTTTACTTCTCTTTCTTGTTGAATCCAAAAGAGTTTGAAAATTTGTTGCAACTTCCACTGGTCTTGGGACTCTCCGTGAGCGAGGCACTCGAAGAGATTACAGAGATACCATTTAGTCTGAAGTGGCCCAACGACGTTTATTTTCAGGAGAAAAAGGTTTCAGGGGTCCTTTGTGAGCTCAGTAAGGACAAGCTGATAGTGGGAATCGGTATAAACGTGAATCAAAGAGAGATCCCTGAAGAGATCAAGGATCGGGCCACAACACTTTATGAAATCACTGGAAAGGATTGGGACAGAAAGGAGGTCTTGCTGAAGGTTCTCAAGCGAATTTCCGAAAATCTCAAGAAATTTAAGGAAAAATCCTTTAAAGAGTTCAAAGGAAAAATTGAGTCAAAGATGTTGTACCTTGGAGAGGAGGTTAAACTCTTGGGAGAAGGCAAGATTACCGGGAAACTTGTGGGCCTGAGTGAAAAGGGCGGTGCTTTGATACTTACAGAGGAAGGCATAAAGGAGATACTGTCCGGCGAATTTAGCTTGCGACGGTCA
Protein Sequence
MFKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWLSQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVSGVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISENLKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEILSGEFSLRRS
|
|||||
| BioID2 | — | promiscuous R40G mutant of a biotin pro… | 3400..4099 | Fwd | |
|
DNA Sequence
ATGTTCAAAAATCTTATTTGGCTGAAGGAAGTTGATAGCACCCAGGAGCGGCTTAAGGAATGGAATGTCTCCTATGGCACTGCACTCGTCGCCGATAGACAGACCAAAGGTAGAGGCGGTCTCGGACGGAAGTGGTTGAGTCAGGAGGGCGGTCTTTACTTCTCTTTCTTGTTGAATCCAAAAGAGTTTGAAAATTTGTTGCAACTTCCACTGGTCTTGGGACTCTCCGTGAGCGAGGCACTCGAAGAGATTACAGAGATACCATTTAGTCTGAAGTGGCCCAACGACGTTTATTTTCAGGAGAAAAAGGTTTCAGGGGTCCTTTGTGAGCTCAGTAAGGACAAGCTGATAGTGGGAATCGGTATAAACGTGAATCAAAGAGAGATCCCTGAAGAGATCAAGGATCGGGCCACAACACTTTATGAAATCACTGGAAAGGATTGGGACAGAAAGGAGGTCTTGCTGAAGGTTCTCAAGCGAATTTCCGAAAATCTCAAGAAATTTAAGGAAAAATCCTTTAAAGAGTTCAAAGGAAAAATTGAGTCAAAGATGTTGTACCTTGGAGAGGAGGTTAAACTCTTGGGAGAAGGCAAGATTACCGGGAAACTTGTGGGCCTGAGTGAAAAGGGCGGTGCTTTGATACTTACAGAGGAAGGCATAAAGGAGATACTGTCCGGCGAATTTAGCTTGCGACGGTCA
Protein Sequence
MFKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWLSQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVSGVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISENLKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEILSGEFSLRRS
|
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| HA | — | HA (human influenza hemagglutinin) epit… | 4108..4135 | Fwd | |
|
DNA Sequence
TACCCTTACGATGTACCGGATTACGCA
Protein Sequence
YPYDVPDYA
|
|||||
| Factor Xa site | — | Factor Xa recognition and cleavage site | 4630..4642 | Rev | |
|
DNA Sequence
ATTGAGGGCCGA
Protein Sequence
IEGR
|
|||||
| AmpR | bla | beta-lactamase | 5986..6847 | Fwd | |
|
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| chimeric intron | — | — | 1238..1371 | Fwd | |
|
DNA Sequence
GTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| WPRE | — | — | 4159..4748 | Fwd | |
|
DNA Sequence
AATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAATCATCGTCCTTTCCTTGGCTGCTCGCCTATGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGC
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| SV40 poly(A) signal | — | — | 4811..4933 | Fwd | |
|
DNA Sequence
TAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac promoter | — | — | 142..173 | Fwd | |
|
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
|
|||||
| T7 promoter | — | — | 1415..1434 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| AmpR promoter | bla | — | 5881..5986 | Fwd | |
|
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| CAP binding site | — | — | 106..128 | Fwd | |
|
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
|
|||||
| lac operator | — | — | 180..197 | Fwd | |
|
DNA Sequence
TTGTGAGCGGATAACAA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| f1 ori | — | — | 5400..5856 | Fwd | |
|
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTT
|
|||||
| ori | — | — | 7017..7606 | Fwd | |
|
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
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