pAT-WT-HA-GFP11

177719 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP
Depositor
Erik Böttger
Article
Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
Insert
alpha-1-antitrypsin (SERPINA1 Human)
Tags
GFP11 and HA
Available Since
Jan. 26, 2022
Availability
Available

Sequence Information

Length
6,261 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 4
Label Gene Product Position Strand
HygR aph(4)-Ia aminoglycoside phosphotransferase from … 140..1178 Fwd
DNA Sequence
ATGAAGAAGCCCGAACTCACCGCTACCAGCGTTGAAAAATTTCTCATCGAGAAGTTCGACAGTGTGAGCGACCTGATGCAGTTGTCGGAGGGCGAAGAGAGCCGAGCCTTCAGCTTCGATGTCGGCGGACGCGGCTATGTACTGCGGGTGAATAGCTGCGCTGATGGCTTCTACAAAGACCGCTACGTGTACCGCCACTTCGCCAGCGCTGCACTACCCATCCCCGAAGTGTTGGACATCGGCGAGTTCAGCGAGAGCCTGACATACTGCATCAGTAGACGCGCCCAAGGCGTTACTCTCCAAGACCTCCCCGAAACAGAGCTGCCTGCTGTGTTACAGCCTGTCGCCGAAGCTATGGATGCTATTGCCGCCGCCGACCTCAGTCAAACCAGCGGCTTCGGCCCATTCGGGCCCCAAGGCATCGGCCAGTACACAACCTGGCGGGATTTCATTTGCGCCATTGCTGATCCCCATGTCTACCACTGGCAGACCGTGATGGACGACACCGTGTCCGCCAGCGTAGCTCAAGCCCTGGACGAACTGATGCTGTGGGCCGAAGACTGTCCCGAGGTGCGCCACCTCGTCCATGCCGACTTCGGCAGCAACAACGTCCTGACCGACAACGGCCGCATCACCGCCGTAATCGACTGGTCCGAAGCTATGTTCGGGGACAGTCAGTACGAGGTGGCCAACATCTTCTTCTGGCGGCCCTGGCTGGCTTGCATGGAGCAGCAGACTCGCTACTTCGAGCGCCGGCATCCCGAGCTGGCCGGCAGCCCTCGTCTGCGAGCCTACATGCTGCGCATCGGCCTGGATCAGCTCTACCAGAGCCTCGTGGACGGCAACTTCGACGATGCTGCCTGGGCTCAAGGCCGCTGCGATGCCATCGTCCGCAGCGGGGCCGGCACCGTCGGTCGCACACAAATCGCTCGCCGGAGCGCAGCCGTATGGACCGACGGCTGCGTCGAGGTGCTGGCCGACAGCGGCAACCGCCGGCCCAGTACACGACCGCGCGCTAAGGAGGTAGGTCGAGTTTAA
Protein Sequence
MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKEVGRV
AmpR bla beta-lactamase 2364..3225 Rev
DNA Sequence
ATGAGCATCCAACATTTTCGTGTCGCACTCATTCCCTTCTTTGCGGCATTTTGCTTGCCTGTTTTTGCACACCCCGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAACTGGGTGCAAGAGTGGGCTATATCGAACTGGATCTCAATAGCGGCAAGATCCTTGAGTCTTTCCGCCCCGAAGAACGATTCCCGATGATGAGCACTTTCAAAGTACTGCTATGTGGCGCGGTGTTGTCCCGTATAGACGCCGGGCAAGAGCAGCTTGGTCGCCGTATACACTACTCACAAAACGACTTGGTTGAGTACTCGCCGGTCACGGAAAAGCATCTTACGGATGGCATGACGGTAAGAGAATTGTGTAGTGCTGCCATTACCATGAGCGACAACACCGCGGCCAACTTACTTCTGACAACGATCGGAGGCCCTAAGGAGCTGACTGCATTTCTTCATAATATGGGTGATCATGTGACCCGGCTTGACCGCTGGGAACCAGAGTTGAACGAAGCCATACCGAACGACGAGCGTGATACCACGATGCCAGTAGCAATGGCCACAACTCTTCGGAAACTACTCACTGGCGAACTTCTTACTCTAGCATCACGACAGCAGCTCATAGACTGGATGGAGGCGGACAAAGTAGCAGGACCACTTCTTCGCTCGGCCCTCCCTGCTGGCTGGTTCATTGCTGACAAATCGGGGGCCGGTGAACGCGGCTCTCGCGGCATCATTGCTGCGCTGGGGCCTGATGGTAAGCCCTCACGAATCGTAGTGATCTACACGACGGGGAGTCAGGCCACTATGGACGAACGAAATAGGCAGATCGCTGAGATCGGTGCCTCACTGATCAAGCACTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
HA — HA (human influenza hemagglutinin) epit… 5538..5565 Fwd
DNA Sequence
TACCCATACGATGTTCCAGATTACGCT
Protein Sequence
YPYDVPDYA
GFP11 — 11th beta-strand of superfolder GFP 5604..5652 Fwd
DNA Sequence
AGAGATCATATGGTTTTGCATGAATATGTTAATGCTGCTGGTATTACT
Protein Sequence
RDHMVLHEYVNAAGIT
RBS 1
Label Gene Product Position Strand
Shine-Dalgarno sequence — — 1156..1165 Fwd
DNA Sequence
TAAGGAGGT
enhancer 2
Label Gene Product Position Strand
CMV enhancer — — 3633..4013 Fwd
DNA Sequence
GGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATG
SV40 enhancer — — 6013..6205 Fwd
DNA Sequence
CTGAGGCGGAAAGAACCAGCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCC
polyA_signal 1
Label Gene Product Position Strand
SV40 poly(A) signal — — 5698..5820 Fwd
DNA Sequence
TAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
promoter 2
Label Gene Product Position Strand
SV40 promoter — — 0..6261 Fwd
DNA Sequence
CTGAGGCGGAAAGAACCAGCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAA
CMV promoter — — 4013..4217 Fwd
DNA Sequence
GTGATGCGGTTTTGGCAGTACACCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCCCGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT
rep_origin 2
Label Gene Product Position Strand
SV40 ori — — 0..6261 Fwd
DNA Sequence
ATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCC
ori — — 1576..2165 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA