p40 E259A

19023 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Michael Yaffe
Article
Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19
Insert
p40phox (NCF4 Human)
Tags
His
Mutation
E259A
Available Since
Aug. 25, 2008
Availability
Available

Sequence Information

Length
823 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 64..82 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 91..109 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
T7 tag (gene 10 leader) — leader peptide from bacteriophage T7 ge… 112..145 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG