pDSP31

199373 Caenorhabditis elegans Bacterial Expression

Plasmid Information

Purpose
Encodes dual cistronic nuclear localized GCaMP6f and mScarlet-I optimized for C. elegans expression
Depositor
Hang Lu
Insert
NLS::GCaMP6f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
Mutation
no mutations, except added subcellular localizati…
Available Since
April 25, 2023
Availability
Available

Sequence Information

Length
5,387 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 6
Label Gene Product Position Strand
SV40 NLS — nuclear localization signal of SV40 (si… 226..247 Fwd
DNA Sequence
CCAAAGAAGAAGCGTAAGGTC
Protein Sequence
PKKKRKV
6xHis — 6xHis affinity tag 262..280 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
Xpress(TM) tag — Xpress(TM) epitope tag, including an en… 319..343 Fwd
DNA Sequence
GACCTTTACGATGACGACGACAAG
Protein Sequence
DLYDDDDK
calmodulin-binding peptide — calmodulin-binding peptide from avian s… 370..427 Fwd
DNA Sequence
CGCCGCAAGTGGAACAAGACTGGACATGCTGTCCGTGCCATCGGACGTCTTTCCTCC
Protein Sequence
RRKWNKTGHAVRAIGRLSS
SV40 NLS — nuclear localization signal of SV40 (si… 2121..2142 Fwd
DNA Sequence
CCAAAGAAGAAGCGTAAGGTC
Protein Sequence
PKKKRKV
KanR aph(3')-Ia aminoglycoside phosphotransferase 4373..5189 Rev
DNA Sequence
ATGAGCCATATTCAACGGGAAACGTCTTGCTTGAAGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGGCTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCAGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACGGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGGTGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTCTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCACTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
promoter 1
Label Gene Product Position Strand
cat promoter — — 5189..5292 Rev
DNA Sequence
TGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAA
rep_origin 1
Label Gene Product Position Strand
ori — — 3614..4203 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGACAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
tbb-2 terminator tbb-2 — 3086..3410 Fwd
DNA Sequence
ATGCAAAATCCTTTCAAGCATTCCCTTCTTCTCTATCACTCTTCTTTCTTTTTGTCAAAAAATTCTCTCGCTAATTTATTTGCTTTTTTAATGTTATTATTTTATGACTTTTTATAGTCACTGAAAAGTTTGCATCTGAGTGAAGTGAATGCTATCAAAATGTGATTCTGTCTGATGTACTTTCACAATCTCTCTTCAATTCCATTTTGAAGTGCTTTAAACCCGAAAGGTTGAGAAAAATGCGAGCGCTCAAATATTTGTATTGTGTTCGTTGAGTGACCCAACAAAAAGAGGAAACTTTATTGTGCCGCCAAGAAAAAAGTC