AAVTRE3_smFP_FLAG

201210 Synthetic Bacterial Expression

Plasmid Information

Purpose
AAV vector for TRE(TRE3G)-driven smFP_FLAG (FLAG-tagged spaghetti monster) expression
Depositor
Tetsuo Yamamori
Article
Watakabe et al J Vis Exp. 2025 Jan 17;(215). doi: 10.37
Insert
smFP _FLAG (from addgene #59756)
Promoter
TRE3G
Available Since
Aug. 14, 2025
Availability
Available

Sequence Information

Length
5,489 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 12
Label Gene Product Position Strand
FLAG — FLAG(R) epitope tag, followed by an ent… 552..576 Fwd
DNA Sequence
GACTACAAGGACGACGACGATAAA
Protein Sequence
DYKDDDDK
FLAG — FLAG(R) epitope tag, followed by an ent… 579..603 Fwd
DNA Sequence
GATTACAAAGATGACGATGATAAG
Protein Sequence
DYKDDDDK
FLAG — FLAG(R) epitope tag, followed by an ent… 606..630 Fwd
DNA Sequence
GATTATAAGGACGACGATGACAAG
Protein Sequence
DYKDDDDK
FLAG — FLAG(R) epitope tag, followed by an ent… 1158..1182 Fwd
DNA Sequence
GACTATAAGGATGACGATGACAAA
Protein Sequence
DYKDDDDK
FLAG — FLAG(R) epitope tag, followed by an ent… 1188..1212 Fwd
DNA Sequence
GACTATAAGGACGATGATGACAAA
Protein Sequence
DYKDDDDK
FLAG — FLAG(R) epitope tag, followed by an ent… 1224..1248 Fwd
DNA Sequence
GATTACAAGGACGATGACGATAAG
Protein Sequence
DYKDDDDK
FLAG — FLAG(R) epitope tag, followed by an ent… 1254..1278 Fwd
DNA Sequence
GATTACAAGGATGATGATGATAAG
Protein Sequence
DYKDDDDK
FLAG — FLAG(R) epitope tag, followed by an ent… 1488..1512 Fwd
DNA Sequence
GATTATAAAGATGACGACGACAAG
Protein Sequence
DYKDDDDK
FLAG — FLAG(R) epitope tag, followed by an ent… 1515..1539 Fwd
DNA Sequence
GACTACAAAGATGATGATGACAAG
Protein Sequence
DYKDDDDK
FLAG — FLAG(R) epitope tag, followed by an ent… 1542..1566 Fwd
DNA Sequence
GACTACAAAGACGACGATGATAAA
Protein Sequence
DYKDDDDK
Factor Xa site — Factor Xa recognition and cleavage site 2086..2098 Rev
DNA Sequence
ATTGAGGGCCGA
Protein Sequence
IEGR
AmpR bla beta-lactamase 3808..4669 Fwd
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
misc_feature 1
Label Gene Product Position Strand
WPRE — — 1615..2204 Fwd
DNA Sequence
AATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAATCATCGTCCTTTCCTTGGCTGCTCGCCTATGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGC
polyA_signal 1
Label Gene Product Position Strand
hGH poly(A) signal — — 2235..2712 Fwd
DNA Sequence
GGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTT
promoter 2
Label Gene Product Position Strand
TRE3G promoter — — 142..521 Fwd
DNA Sequence
GAGTTTACTCCCTATCAGTGATAGAGAACGTATGAAGAGTTTACTCCCTATCAGTGATAGAGAACGTATGCAGACTTTACTCCCTATCAGTGATAGAGAACGTATAAGGAGTTTACTCCCTATCAGTGATAGAGAACGTATGACCAGTTTACTCCCTATCAGTGATAGAGAACGTATCTACAGTTTACTCCCTATCAGTGATAGAGAACGTATATCCAGTTTACTCCCTATCAGTGATAGAGAACGTATAAGCTTTAGGCGTGTACGGTGGGCGCCTATAAAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGCAATTCCACAACACTTTTGTCTTATACCAACTTTCCGTACCACTTCCTACCCTCGTAAA
AmpR promoter bla — 3703..3808 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 7
Label Gene Product Position Strand
tet operator tetO — 150..169 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 186..205 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 222..241 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 258..277 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 294..313 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 330..349 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 366..385 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
rep_origin 2
Label Gene Product Position Strand
f1 ori — — 2966..3422 Fwd
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCTTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACTCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGTCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTT
ori — — 4839..5428 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA