| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| KanR | aph(3')-Ia | aminoglycoside phosphotransferase | 551..1361 | Rev | |
|
DNA Sequence
ATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCACTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCGTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCACTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGCCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAATTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
|
|||||
| 8xHis | — | 8xHis affinity tag | 1708..1732 | Fwd | |
|
DNA Sequence
CACCATCACCATCATCATCATCAC
Protein Sequence
HHHHHHHH
|
|||||
| SV40 NLS | — | nuclear localization signal of SV40 (si… | 1765..1786 | Fwd | |
|
DNA Sequence
CCGAAAAAAAAGCGTAAAGTT
Protein Sequence
PKKKRKV
|
|||||
| UGI | — | uracil-DNA glycosylase inhibitor from a… | 6517..6766 | Fwd | |
|
DNA Sequence
ACCAACCTGAGCGACATTATTGAAAAAGAAACCGGCAAACAACTGGTCATCCAAGAAAGCATTCTGATGTTGCCGGAGGAAGTTGAAGAGGTGATTGGCAATAAACCGGAGAGCGATATCCTGGTGCATACCGCGTACGACGAAAGCACTGATGAGAATGTGATGTTGCTGACCTCCGATGCACCGGAGTACAAACCGTGGGCGTTGGTGATCCAAGATAGCAATGGTGAAAACAAGATTAAGATGCTG
Protein Sequence
TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML
|
|||||
| UGI | — | uracil-DNA glycosylase inhibitor from a… | 6796..7045 | Fwd | |
|
DNA Sequence
ACCAACTTGTCCGACATCATTGAGAAAGAAACCGGTAAGCAGCTGGTTATTCAAGAATCTATTCTGATGCTGCCGGAAGAGGTTGAAGAAGTCATCGGCAATAAGCCTGAGAGCGACATTTTGGTTCATACCGCTTACGATGAGAGCACGGATGAAAATGTAATGCTGCTGACGAGCGACGCTCCGGAGTACAAGCCGTGGGCACTGGTGATTCAAGATAGCAACGGCGAGAATAAGATCAAAATGCTG
Protein Sequence
TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML
|
|||||
| SV40 NLS | — | nuclear localization signal of SV40 (si… | 7090..7111 | Fwd | |
|
DNA Sequence
CCGAAAAAGAAACGCAAGGTG
Protein Sequence
PKKKRKV
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| cat promoter | — | — | 1361..1452 | Rev | |
|
DNA Sequence
TGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCT
|
|||||
| rhaB promoter | — | — | 1555..1674 | Fwd | |
|
DNA Sequence
CACCACAATTCAGCAAATTGTGAACATCATCACGTTCATCTTTCCCTGGTTGCCAATGGCCCATTTTCCTGTCAGTAACGAGAAGGTCGCGAATTCAGGCGCTTTTTAGACTGGTCGTA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| p15A ori | — | — | 0..7659 | Fwd | |
|
DNA Sequence
TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7Te terminator | — | — | 500..528 | Rev | |
|
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG
|
|||||
| tonB terminator | — | — | 1460..1491 | Rev | |
|
DNA Sequence
AGTCAAAAGCCTCCGACCGGAGGCTTTTGAC
|
|||||
| T7 terminator | — | — | 7145..7193 | Fwd | |
|
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
|
|||||
| T3Te terminator | — | — | 7290..7320 | Fwd | |
|
DNA Sequence
GGCTCACCTTCACGGGTGGGCCTTTCTTCG
|
|||||