pAMC070n1a12

209746 Synthetic Bacterial Expression

Plasmid Information

Purpose
ColA BBa_B0014 tetAp SD8 ScTrpRS rrnB1 proB SD8 Lum1PylRS BBaB10014RNAItrpA+ J23100 SD8 MmPylRS T3Te-T7Te DHFR
Depositor
Ahmed Badran
Article
Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103
Insert
tRNA operon - ScTrpRS - Lum1PylRS - MmPylRS
Available Since
Oct. 18, 2024
Availability
Available

Sequence Information

Length
6,621 bp
Topology
circular
Molecule Type
other DNA

Features

promoter 2
Label Gene Product Position Strand
tetR/tetA promoters tetR — 1711..1767 Fwd
DNA Sequence
GTTGACACTCTATCATTGATAGAGTTATTTTACCACTCCCTATCAGTGATAGAGAA
BBa_J23100 — — 4420..4455 Fwd
DNA Sequence
TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC
protein_bind 3
Label Gene Product Position Strand
lac operator — — 930..947 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
tet operator tetO — 1717..1736 Fwd
DNA Sequence
ACTCTATCATTGATAGAGT
tet operator tetO — 1747..1766 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
rep_origin 1
Label Gene Product Position Strand
ColA ori — — 0..636 Rev
DNA Sequence
TGGTGTCGGGAATCCGTAAATGATCTTCTTGAGATCCTTTTACGATCGTCGTAATCTCCTGCTCTGTAAACGAAAAAACCGCCTGGGGAGGCGGTTTGATCGAAGGTTAAGTCAGTTGGGGAACTGCTTAACCTGGTAACTGGCTTTAGTGGAGCGCAGATACCAAATACTGTCCTTTCAGTGTAGCCTCTGTTAGGCCACCACTTCAAGACTCTCGATATCTAAATCCACTAATTCTCAGTTACCAATGGCTGCTGCCAGTGGCGTTTTGTCGTGTCTTTCCGGGTTGGACTCAAGATGATAGTTACCGGATAAGGCGCAGCAGTCGGGCTGAACGGGGGGTTCTTGCACACAGCCCAGCTTGGAGCGAACTGTCTACACGGAACGGGACGTGGTGATTTGGGTAAAGCCTCCACCACAACACGGACGCCGCAGGACGGGAACAGGAGAGCGCAAGAGGGAGCCATCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGTTCGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTCGCTCCGGCCTTATTGTCTCTCTGCTAAGTATCCTCCTGGCATCTTCTAGGACGTTT
terminator 4
Label Gene Product Position Strand
T7Te terminator — — 658..686 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG
T7Te terminator — — 4225..4253 Fwd
DNA Sequence
GGCTCAACTTCGGGTGGGCCTTTCTGCG
T3Te terminator — — 5882..5912 Fwd
DNA Sequence
GGCTCACCTTCACGGGTGAGCCTTTCTTCG
T7Te terminator — — 5912..5940 Rev
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG