| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| UGI | — | uracil-DNA glycosylase inhibitor from a… | 1335..1584 | Fwd | |
|
DNA Sequence
ACCAACCTGAGCGACATTATTGAAAAAGAAACCGGCAAACAACTGGTCATCCAAGAAAGCATTCTGATGTTGCCGGAGGAAGTTGAAGAGGTGATTGGCAATAAACCGGAGAGCGATATCCTGGTGCATACCGCGTACGACGAAAGCACTGATGAGAATGTGATGTTGCTGACCTCCGATGCACCGGAGTACAAACCGTGGGCGTTGGTGATCCAAGATAGCAATGGTGAAAACAAGATTAAGATGCTG
Protein Sequence
TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML
|
|||||
| UGI | — | uracil-DNA glycosylase inhibitor from a… | 1614..1863 | Fwd | |
|
DNA Sequence
ACCAACTTGTCCGACATCATTGAGAAAGAAACCGGTAAGCAGCTGGTTATTCAAGAATCTATTCTGATGCTGCCGGAAGAGGTTGAAGAAGTCATCGGCAATAAGCCTGAGAGCGACATTTTGGTTCATACCGCTTACGATGAGAGCACGGATGAAAATGTAATGCTGCTGACGAGCGACGCTCCGGAGTACAAGCCGTGGGCACTGGTGATTCAAGATAGCAACGGCGAGAATAAGATCAAAATGCTG
Protein Sequence
TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML
|
|||||
| SV40 NLS | — | nuclear localization signal of SV40 (si… | 1908..1929 | Fwd | |
|
DNA Sequence
CCGAAAAAGAAACGCAAGGTG
Protein Sequence
PKKKRKV
|
|||||
| KanR | aph(3')-Ia | aminoglycoside phosphotransferase | 3022..3832 | Rev | |
|
DNA Sequence
ATGAGCAATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCACTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCGTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCACTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGCCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAATTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSNIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
|
|||||
| 8xHis | — | 8xHis affinity tag | 4179..4203 | Fwd | |
|
DNA Sequence
CACCATCACCATCATCATCATCAC
Protein Sequence
HHHHHHHH
|
|||||
| SV40 NLS | — | nuclear localization signal of SV40 (si… | 4236..4257 | Fwd | |
|
DNA Sequence
CCGAAAAAAAAGCGTAAAGTT
Protein Sequence
PKKKRKV
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| cat promoter | — | — | 3832..3923 | Rev | |
|
DNA Sequence
TGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCT
|
|||||
| rhaB promoter | — | — | 4026..4145 | Fwd | |
|
DNA Sequence
CACCACAATTCAGCAAATTGTGAACATCATCACGTTCATCTTTCCCTGGTTGCCAATGGCCCATTTTCCTGTCAGTAACGAGAAGGTCGCGAATTCAGGCGCTTTTTAGACTGGTCGTA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| p15A ori | — | — | 2161..2707 | Fwd | |
|
DNA Sequence
TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 terminator | — | — | 1957..2005 | Fwd | |
|
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
|
|||||
| T3Te terminator | — | — | 2102..2132 | Fwd | |
|
DNA Sequence
GGCTCACCTTCACGGGTGGGCCTTTCTTCG
|
|||||
| T7Te terminator | — | — | 2971..2999 | Rev | |
|
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG
|
|||||
| tonB terminator | — | — | 3931..3962 | Rev | |
|
DNA Sequence
AGTCAAAAGCCTCCGACCGGAGGCTTTTGAC
|
|||||