pEND-375_Cacnes_phlF

225620 Synthetic Bacterial Expression

Plasmid Information

Purpose
C. acnes with PhlF-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+PhlF // P(BBa_23119-PhlO1O3)+RBS_1+mCherry.
Depositor
Marc Güell
Article
Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
Insert
PhlF
Tags
6xHis and mCherry-V5-6xHis
Mutation
Codon optimized for Cutibacterium acnes
Available Since
Feb. 6, 2025
Availability
Available

Sequence Information

Length
7,945 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 5
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 1047..1065 Fwd
DNA Sequence
CACCACCACCACCATCAC
Protein Sequence
HHHHHH
Factor Xa site — Factor Xa recognition and cleavage site 2337..2349 Fwd
DNA Sequence
ATCGAGGGCCGC
Protein Sequence
IEGR
V5 tag — epitope tag from simian virus 5 2349..2391 Fwd
DNA Sequence
GGTAAGCCGATCCCGAACCCGCTGTTGGGCCTGGATTCCACT
Protein Sequence
GKPIPNPLLGLDST
6xHis — 6xHis affinity tag 2400..2418 Fwd
DNA Sequence
CACCATCACCACCACCAC
Protein Sequence
HHHHHH
AmpR bla beta-lactamase 5525..6386 Rev
DNA Sequence
ATGTCGATCCAGCACTTCCGCGTTGCCCTTATCCCATTTTTTGCGGCCTTTTGCTTGCCGGTTTTTGCCCATCCGGAAACCCTGGTCAAGGTCAAGGACGCCGAGGATCAGCTGGGGGCGCGCGTCGGTTATATCGAGTTGGACCTGAACTCCGGTAAGATTCTGGAGTCTTTCCGCCCTGAGGAGCGTTTCCCCATGATGTCCACGTTCAAGGTCCTCCTCTGCGGCGCTGTGCTTAGCCGGGTGGATGCTGGGCAGGAGCAACTGGGCCGTCGCATTCATTACTCCCAGAACGACCTGGTCGAATACTCCCCGGTCACGGAGAAACATCTGACCGATGGGATGACCGTGCGCGAGCTGTGCTCCGCGGCCATCACTATGTCCGACAACACTGCCGCCAATCTGCTGTTGACGACCATCGGTGGCCCGAAAGAGCTGACGGCTTTTCTCCACAACATGGGCGACCACGTGACCCGTCTGGACCGTTGGGAACCCGAACTTAATGAGGCTATCCCGAACGACGAGCGCGACACGACTATGCCCGCGGCTATGGCTACCACCCTCCGTAAACTCCTGACCGGTGAACTCCTCACTCTGGCCTCCCGTCAGCAACTGATCGACTGGATGGAGGCCGACAAGGTCGCTGGCCCGCTGTTGCGCAGCGCGCTCCCGGCCGGTTGGTTCATCGCGGACAAGTCGGGTGCTGGTGAGCGCGGCTCGCGTGGCATCATCGCGGCTCTCGGTCCCGACGGTAAGCCCAGCCGCATCGTGGTGATCTACACGACCGGCTCCCAGGCCACGATGGATGAGCGCAACCGTCAGATCGCCGAGATCGGTGCCTCCCTCATCAAGCATTGGTGA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
promoter 2
Label Gene Product Position Strand
J23119 promoter — — 1469..1504 Fwd
DNA Sequence
TTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGC
AmpR promoter bla — 6386..6491 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
rep_origin 1
Label Gene Product Position Strand
ori — — 4854..5443 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA