| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| FLAG | — | FLAG(R) epitope tag, followed by an ent… | 191..215 | Fwd | |
|
DNA Sequence
GATTACAAAGATGACGATGACAAA
Protein Sequence
DYKDDDDK
|
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| 6xHis | — | 6xHis affinity tag | 3160..3178 | Fwd | |
|
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
|
|||||
| KanR | aph(3')-Ia | aminoglycoside phosphotransferase | 8911..9727 | Rev | |
|
DNA Sequence
ATGAGCCATATTCAACGGGAAACGTCTTGCTCTAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGCAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGACCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
|
|||||
| lacI | lacI | lac repressor | 10666..11749 | Rev | |
|
DNA Sequence
GTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGACATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGA
Protein Sequence
MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lacI promoter | lacI | — | 0..11779 | Rev | |
|
DNA Sequence
GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT
|
|||||
| T7 promoter | — | — | 98..117 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| T7 promoter | — | — | 1646..1665 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| T7 promoter | — | — | 3061..3080 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| T7 promoter | — | — | 4491..4510 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| T7 promoter | — | — | 5899..5918 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| T7 promoter | — | — | 7220..7239 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| AmpR promoter | bla | — | 9727..9819 | Rev | |
|
DNA Sequence
TTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 117..142 | Fwd | |
|
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
|
|||||
| lac operator | — | — | 1665..1690 | Fwd | |
|
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
|
|||||
| lac operator | — | — | 3080..3105 | Fwd | |
|
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
|
|||||
| lac operator | — | — | 4510..4535 | Fwd | |
|
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
|
|||||
| lac operator | — | — | 5918..5943 | Fwd | |
|
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
|
|||||
| lac operator | — | — | 7239..7264 | Fwd | |
|
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| ColA ori | — | — | 9836..10469 | Rev | |
|
DNA Sequence
TGGTGTCGGGAATCCGTAAAGGATCTTCTTGAGATCCTTTTATCGTCGTAATATCCTGCTCTGTAAACGAAAAAACCGCCTGGGGAGGCGGTTTGATCGAAGGTTAAGTCAGTTGGGGAACTGCTTAACCTGGTAACTGGCTTTAGTGGAGCGCAGATACCAAATACTGTCCTTTCAGTGTAGCCTCTGTTAGGCCACCACTTCAAGACTCTCGATATCTAAATCCACTAATTCTCAGTTACCAATGGCTGCTGCCAGTGGCGTTTTGTCGTGTCTTTCCGGGTTGGACTCAAGATGATAGTTACCGGATAAGGCGCAGCAGTCGGGCTGAACGGGGGGTTCTTGCACACAGCCCAGCTTGGAGCGAACTGTCTACACGGAACGGGACGTGGTGATTTGGGTAAAGCCTCCACCACAACACGGACGCCGCAGGACGGGAACAGGAGAGCGCAAGAGGGAGCCATCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGTTCGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTCGCTCCGGCCTTATTGTCTCTCTGCTAAGTATCCTCCTGGCATCTTCTAGGACGTTT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 terminator | — | — | 8640..8688 | Fwd | |
|
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
|
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