| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| AmpR | bla | beta-lactamase | 204..1065 | Fwd | |
|
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
|
|||||
| EGFP | — | the original enhanced GFP (Yang et al.,… | 4674..5391 | Fwd | |
|
DNA Sequence
ATGGTTTCTAAGGGTGAAGAGTTGTTCACTGGTGTTGTTCCAATCTTGGTTGAGTTGGACGGTGACGTTAACGGACACAAGTTCTCTGTTTCTGGTGAAGGTGAGGGTGACGCTACTTACGGAAAGTTGACTTTGAAGTTCATCTGTACTACTGGTAAGTTGCCAGTTCCATGGCCAACTTTGGTTACTACTTTGACTTACGGTGTTCAGTGTTTCTCCAGATACCCAGACCACATGAAGCAGCACGATTTCTTCAAGTCTGCTATGCCAGAGGGTTACGTTCAAGAGAGAACTATCTTCTTCAAGGACGACGGTAACTACAAGACTAGAGCTGAGGTTAAGTTCGAGGGTGACACATTGGTTAACAGAATCGAGTTGAAGGGTATCGACTTCAAAGAGGACGGAAACATCTTGGGTCACAAGTTGGAGTACAACTACAACTCCCACAACGTTTACATCATGGCTGACAAGCAGAAGAACGGTATCAAGGTTAACTTCAAGATCAGACACAACATCGAGGACGGTTCCGTTCAATTGGCTGACCACTACCAACAGAACACTCCAATTGGTGACGGTCCAGTTTTGTTGCCAGACAACCACTACTTGTCCACTCAATCCGCTTTGTCCAAGGACCCAAACGAGAAGAGAGATCACATGGTTTTGTTGGAGTTCGTTACTGCTGCTGGTATCACTTTGGGTATGGACGAGTTGTACAAG
Protein Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
|
|||||
| HygR | aph(4)-Ia | aminoglycoside phosphotransferase from … | 6111..7134 | Fwd | |
|
DNA Sequence
ATGAAGAAGCCAGAGTTGACTGCTACTTCCGTTGAGAAGTTCTTGATCGAGAAGTTCGACTCCGTTTCCGACTTGATGCAATTGTCTGAAGGTGAAGAGTCCAGAGCTTTCTCCTTTGACGTTGGTGGTAGAGGTTACGTTTTGAGAGTTAATTCCTGTGCTGACGGTTTCTACAAGGACAGATACGTTTACAGACACTTCGCTTCCGCTGCTTTGCCAATTCCAGAGGTTTTGGACATTGGTGAGTTCTCCGAGTCCTTGACTTACTGTATCTCCAGAAGAGCTCAGGGAGTTACATTGCAAGACTTGCCAGAAACTGAGTTGCCAGCTGTTTTGCAACCAGTTGCTGAAGCTATGGACGCTATTGCTGCTGCTGACTTGTCTCAAACTTCTGGTTTTGGTCCATTCGGTCCACAGGGTATTGGTCAGTACACTACTTGGAGAGACTTCATCTGTGCTATCGCTGACCCACATGTTTACCACTGGCAAACTGTTATGGACGATACTGTTTCCGCTTCCGTTGCTCAGGCTTTGGACGAATTGATGTTGTGGGCTGAAGATTGTCCTGAGGTTAGACATTTGGTTCACGCTGACTTCGGTTCCAACAACGTTTTGACTGACAACGGTAGAATCACTGCTGTTATCGACTGGTCCGAAGCTATGTTCGGTGACTCTCAATACGAGGTTGCTAACATCTTCTTTTGGAGACCATGGTTGGCTTGTATGGAACAGCAGACTAGATACTTCGAGAGAAGACACCCAGAATTGGCTGGTTCCCCAAGATTGAGAGCTTACATGTTGAGAATCGGTTTGGACCAGTTGTACCAGTCCTTGGTTGATGGTAACTTCGATGATGCTGCTTGGGCTCAAGGTAGATGTGACGCTATCGTTAGATCCGGTGCTGGTACTGTTGGTAGAACTCAGATTGCTAGAAGATCCGCTGCTGTTTGGACTGACGGTTGTGTTGAAGTTTTGGCTGACTCCGGTAACAGAAGACCATCCACTAGACCAAGAGCTAAAGAG
Protein Sequence
MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| AmpR promoter | bla | — | 99..204 | Fwd | |
|
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
|
|||||
| lac promoter | — | — | 2147..2178 | Fwd | |
|
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| CAP binding site | — | — | 2111..2133 | Fwd | |
|
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
|
|||||
| lac operator | — | — | 2185..2202 | Fwd | |
|
DNA Sequence
TTGTGAGCGGATAACAA
|
|||||
| tet operator | tetO | — | 3959..3978 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 3995..4014 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 4030..4049 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 4066..4085 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 4102..4121 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 4137..4156 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 4173..4192 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 4242..4261 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 4278..4297 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 4313..4332 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 4349..4368 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 4385..4404 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 4420..4439 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 4456..4475 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| ori | — | — | 1235..1824 | Fwd | |
|
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| AOX1 terminator | Pichia pastoris AOX1 | — | 5468..5715 | Fwd | |
|
DNA Sequence
TCAAGAGGATGTCAGAATGCCATTTGCCTGAGAGATGCAGGCTTCATTTTTGATACTTTTTTATTTGTAACCTATATAGTATAGGATTTTTTTTGTCATTTTGTTTCTTCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCTGATGAATATCTTGTGGTAGGGGTTTGGGAAAATCATTCGAGTTTGATGTTTTTCTTGGTATTTCCCACTCCTCTTCAGAGTACAGAAGATTAAGTGAGA
|
|||||