| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| AmpR | bla | beta-lactamase | 200..1061 | Fwd | |
|
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
|
|||||
| EGFP | — | the original enhanced GFP (Yang et al.,… | 4244..4961 | Fwd | |
|
DNA Sequence
ATGGTTTCTAAGGGTGAAGAGTTGTTCACTGGTGTTGTTCCAATCTTGGTTGAGTTGGACGGTGACGTTAACGGACACAAGTTCTCTGTTTCTGGTGAAGGTGAGGGTGACGCTACTTACGGAAAGTTGACTTTGAAGTTCATCTGTACTACTGGTAAGTTGCCAGTTCCATGGCCAACTTTGGTTACTACTTTGACTTACGGTGTTCAGTGTTTCTCCAGATACCCAGACCACATGAAGCAGCACGATTTCTTCAAGTCTGCTATGCCAGAGGGTTACGTTCAAGAGAGAACTATCTTCTTCAAGGACGACGGTAACTACAAGACTAGAGCTGAGGTTAAGTTCGAGGGTGACACATTGGTTAACAGAATCGAGTTGAAGGGTATCGACTTCAAAGAGGACGGAAACATCTTGGGTCACAAGTTGGAGTACAACTACAACTCCCACAACGTTTACATCATGGCTGACAAGCAGAAGAACGGTATCAAGGTTAACTTCAAGATCAGACACAACATCGAGGACGGTTCCGTTCAATTGGCTGACCACTACCAACAGAACACTCCAATTGGTGACGGTCCAGTTTTGTTGCCAGACAACCACTACTTGTCCACTCAATCCGCTTTGTCCAAGGACCCAAACGAGAAGAGAGATCACATGGTTTTGTTGGAGTTCGTTACTGCTGCTGGTATCACTTTGGGTATGGACGAGTTGTACAAG
Protein Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
|
|||||
| HygR | aph(4)-Ia | aminoglycoside phosphotransferase from … | 5681..6704 | Fwd | |
|
DNA Sequence
ATGAAGAAGCCAGAGTTGACTGCTACTTCCGTTGAGAAGTTCTTGATCGAGAAGTTCGACTCCGTTTCCGACTTGATGCAATTGTCTGAAGGTGAAGAGTCCAGAGCTTTCTCCTTTGACGTTGGTGGTAGAGGTTACGTTTTGAGAGTTAATTCCTGTGCTGACGGTTTCTACAAGGACAGATACGTTTACAGACACTTCGCTTCCGCTGCTTTGCCAATTCCAGAGGTTTTGGACATTGGTGAGTTCTCCGAGTCCTTGACTTACTGTATCTCCAGAAGAGCTCAGGGAGTTACATTGCAAGACTTGCCAGAAACTGAGTTGCCAGCTGTTTTGCAACCAGTTGCTGAAGCTATGGACGCTATTGCTGCTGCTGACTTGTCTCAAACTTCTGGTTTTGGTCCATTCGGTCCACAGGGTATTGGTCAGTACACTACTTGGAGAGACTTCATCTGTGCTATCGCTGACCCACATGTTTACCACTGGCAAACTGTTATGGACGATACTGTTTCCGCTTCCGTTGCTCAGGCTTTGGACGAATTGATGTTGTGGGCTGAAGATTGTCCTGAGGTTAGACATTTGGTTCACGCTGACTTCGGTTCCAACAACGTTTTGACTGACAACGGTAGAATCACTGCTGTTATCGACTGGTCCGAAGCTATGTTCGGTGACTCTCAATACGAGGTTGCTAACATCTTCTTTTGGAGACCATGGTTGGCTTGTATGGAACAGCAGACTAGATACTTCGAGAGAAGACACCCAGAATTGGCTGGTTCCCCAAGATTGAGAGCTTACATGTTGAGAATCGGTTTGGACCAGTTGTACCAGTCCTTGGTTGATGGTAACTTCGATGATGCTGCTTGGGCTCAAGGTAGATGTGACGCTATCGTTAGATCCGGTGCTGGTACTGTTGGTAGAACTCAGATTGCTAGAAGATCCGCTGCTGTTTGGACTGACGGTTGTGTTGAAGTTTTGGCTGACTCCGGTAACAGAAGACCATCCACTAGACCAAGAGCTAAAGAG
Protein Sequence
MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| AmpR promoter | bla | — | 95..200 | Fwd | |
|
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
|
|||||
| lac promoter | — | — | 2143..2174 | Fwd | |
|
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| CAP binding site | — | — | 2107..2129 | Fwd | |
|
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
|
|||||
| lac operator | — | — | 2181..2198 | Fwd | |
|
DNA Sequence
TTGTGAGCGGATAACAA
|
|||||
| tet operator | tetO | — | 3955..3974 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 3990..4009 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 4026..4045 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| ori | — | — | 1231..1820 | Fwd | |
|
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| AOX1 terminator | Pichia pastoris AOX1 | — | 5038..5285 | Fwd | |
|
DNA Sequence
TCAAGAGGATGTCAGAATGCCATTTGCCTGAGAGATGCAGGCTTCATTTTTGATACTTTTTTATTTGTAACCTATATAGTATAGGATTTTTTTTGTCATTTTGTTTCTTCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCTGATGAATATCTTGTGGTAGGGGTTTGGGAAAATCATTCGAGTTTGATGTTTTTCTTGGTATTTCCCACTCCTCTTCAGAGTACAGAAGATTAAGTGAGA
|
|||||