pSEL1

23011 Mus musculus Bacterial Expression

Plasmid Information

Depositor
James Bowie
Article
Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.
Insert
DHFR (Dhfr Mouse)
Tags
6 His Tag (not in frame) and Xpress epitope (not …
Available Since
March 10, 2010
Availability
Available

Sequence Information

Length
714 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
Xpress(TM) tag — Xpress(TM) epitope tag, including an en… 312..336 Rev
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK
T7 tag (gene 10 leader) — leader peptide from bacteriophage T7 ge… 339..372 Rev
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
6xHis — 6xHis affinity tag 375..393 Rev
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
araBAD promoter araBAD — 438..604 Rev
DNA Sequence
AAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCAT