| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| mScarlet3 | — | bright cysteine-free monomeric red fluo… | 206..893 | Fwd | |
|
DNA Sequence
ATGGATAGCACCGAGGCAGTGATCAAGGAGTTCATGCGGTTCAAGGTGCACATGGAGGGCTCCATGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCTCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAGGGCCTTCATCAAGCACCCCGCCGACATCCCCGACTACTGGAAGCAGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGATCTTCGAGGACGGCGGCACCGTGTCCGTGACCCAGGACACCTCCCTGGAGGACGGCACCCTGATCTACAAGGTGAAGCTCCGCGGCGGCAACTTCCCTCCTGACGGCCCCGTAATGCAGAAGAGGACAATGGGCTGGGAAGCATCCACCGAGCGGTTGTACCCCGAGGACGTCGTGCTGAAGGGCGACATTAAGATGGCCCTGCGCCTGAAGGACGGCGGCCGCTACCTGGCGGACTTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGATGCCCGGCGCCTTCAACATCGACCGCAAGTTGGACATCACCTCCCACAACGAGGACTACACCGTGGTGGAACAGTACGAACGCTCCGTGGCCCGCCACTCCACCGGCGGCTCCGGTGGCTCC
Protein Sequence
MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGTVSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKMALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHSTGGSGGS
|
|||||
| TetR | tetR | tetracycline repressor TetR | 3504..4128 | Fwd | |
|
DNA Sequence
ATGTCCAGATTAGATAAAAGTAAAGTGATTAACAGCGCATTAGAGCTGCTTAATGAGGTCGGAATCGAAGGTTTAACAACCCGTAAACTCGCCCAGAAGCTAGGTGTAGAGCAGCCTACATTGTATTGGCATGTAAAAAATAAGCGGGCTTTGCTCGACGCCTTAGCCATTGAGATGTTAGATAGGCACCATACTCACTTTTGCCCTTTAGAAGGGGAAAGCTGGCAAGATTTTTTACGTAATAACGCTAAAAGTTTTAGATGTGCTTTACTAAGTCATCGCGATGGAGCAAAAGTACATTTAGGTACACGGCCTACAGAAAAACAGTATGAAACTCTCGAAAATCAATTAGCCTTTTTATGCCAACAAGGTTTTTCACTAGAGAATGCATTATATGCACTCAGCGCTGTGGGGCATTTTACTTTAGGTTGCGTATTGGAAGATCAAGAGCATCAAGTCGCTAAAGAAGAAAGGGAAACACCTACTACTGATAGTATGCCGCCATTATTACGACAAGCTATCGAATTATTTGATCACCAAGGTGCAGAGCCAGCCTTCTTATTCGGCCTTGAATTGATCATATGCGGATTAGAAAAACAACTTAAATGTGAAAGTGGGTCTTAA
Protein Sequence
MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS
|
|||||
| mGreenLantern | — | monomeric GFP derivative that displays … | 4397..5117 | Fwd | |
|
DNA Sequence
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTCCGCGGCGAGGGCGAGGGCGATGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCTTAGGCTACGGCGTGGCCTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTCTTTCAAGGACGACGGTACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGTGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTTCAACAGCCACAAGGTCTATATCACGGCCGACAAGCAGAAGAACGGCATCAAGGCTAACTTCAAGACCCGCCACAACGTTGAGGACGGCGGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCCATCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGAAGGAGAGGGTGACCGCCGCCGGGATTACACATGACATGGACGAGCTGTACAAGTAA
Protein Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIKANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLKERVTAAGITHDMDELYK
|
|||||
| AmpR | bla | beta-lactamase | 7144..8005 | Rev | |
|
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCCCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| tet operator | tetO | — | 1022..1041 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1069..1088 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1118..1137 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1150..1169 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1192..1211 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1238..1257 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1280..1299 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1328..1347 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1377..1396 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1419..1438 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1482..1501 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1529..1548 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1578..1597 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1610..1629 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1652..1671 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1698..1717 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1740..1759 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1788..1807 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1837..1856 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1879..1898 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1942..1961 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 1989..2008 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2038..2057 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2070..2089 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2112..2131 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2158..2177 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2200..2219 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2248..2267 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2297..2316 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2339..2358 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2402..2421 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2449..2468 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2498..2517 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2530..2549 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2572..2591 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2618..2637 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2660..2679 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2708..2727 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2757..2776 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 2799..2818 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 4292..4311 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| R6K gamma ori | — | — | 9296..9685 | Fwd | |
|
DNA Sequence
TGTCAGCCGTTAAGTGTTCCTGTGTCACTCAAAATTGCTTTGAGAGGCTCTAAGGGCTTCTCAGTGCGTTACATCCCTGGCTTGTTGTCCACAACCGTTAAACCTTAAAAGCTTTAAAAGCCTTATATATTCTTTTTTTTCTTATAAAACTTAAAACCTTAGAGGCTATTTAAGTTGCTGATTTATATTAATTTTATTGTTCAAACATGAGAGCTTAGTACGTGAAACATGAGAGCTTAGTACGTTAGCCATGAGAGCTTAGTACGTTAGCCATGAGGGTTTAGTTCGTTAAACATGAGAGCTTAGTACGTTAAACATGAGAGCTTAGTACGTGAAACATGAGAGCTTAGTACGTACTATCAACAGGTTGAACTGCTGATCTTCAGATC
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| rrnB T2 terminator | — | — | 918..946 | Fwd | |
|
DNA Sequence
AGAAGGCCATCCTGACGGATGGCCTTTT
|
|||||
| rrnB T1 terminator | Escherichia coli rrnB | — | 2852..2924 | Fwd | |
|
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
|
|||||
| T7Te terminator | — | — | 2939..2967 | Fwd | |
|
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG
|
|||||
| rrnB T2 terminator | — | — | 5137..5165 | Fwd | |
|
DNA Sequence
AGAAGGCCATCCTGACGGATGGCCTTTT
|
|||||