pEM352

248764 Synthetic Bacterial Expression

Plasmid Information

Purpose
160xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly
Depositor
Carolina Tropini
Insert
T7Te-rrnBT1-160xTetO
Available Since
March 25, 2026
Availability
Available

Sequence Information

Length
9,618 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 1
Label Gene Product Position Strand
KanR aph(3')-Ia aminoglycoside phosphotransferase 7357..8173 Rev
DNA Sequence
ATGAGCCATATTCAACGGGAAACGTCTTGCTCCAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATCGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
protein_bind 155
Label Gene Product Position Strand
tet operator tetO — 38..57 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 85..104 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 134..153 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 166..185 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 208..227 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 254..273 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 296..315 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 344..363 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 393..412 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 435..454 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 498..517 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 545..564 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 594..613 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 626..645 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 668..687 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 714..733 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 756..775 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 804..823 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 853..872 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 895..914 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 958..977 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1005..1024 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1054..1073 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1086..1105 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1128..1147 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1174..1193 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1216..1235 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1264..1283 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1313..1332 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1355..1374 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1418..1437 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1465..1484 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1514..1533 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1546..1565 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1588..1607 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1634..1653 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1676..1695 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1724..1743 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1773..1792 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1815..1834 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1878..1897 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1925..1944 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 1974..1993 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2006..2025 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2048..2067 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2094..2113 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2136..2155 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2184..2203 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2233..2252 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2275..2294 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2321..2340 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2363..2382 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2411..2430 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2460..2479 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2502..2521 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2565..2584 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2612..2631 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2661..2680 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2693..2712 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2735..2754 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2781..2800 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2823..2842 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2871..2890 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2920..2939 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 2962..2981 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3025..3044 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3072..3091 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3121..3140 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3153..3172 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3195..3214 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3241..3260 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3283..3302 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3331..3350 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3380..3399 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3422..3441 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3485..3504 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3532..3551 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3581..3600 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3613..3632 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3655..3674 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3701..3720 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3743..3762 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3791..3810 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3840..3859 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3882..3901 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3945..3964 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 3992..4011 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4041..4060 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4073..4092 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4115..4134 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4161..4180 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4203..4222 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4251..4270 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4300..4319 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4342..4361 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4405..4424 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4452..4471 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4501..4520 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4533..4552 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4575..4594 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4621..4640 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4663..4682 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4711..4730 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4760..4779 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4802..4821 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4865..4884 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4912..4931 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4961..4980 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 4993..5012 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5035..5054 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5081..5100 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5123..5142 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5171..5190 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5220..5239 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5262..5281 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5325..5344 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5372..5391 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5421..5440 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5453..5472 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5495..5514 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5541..5560 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5583..5602 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5631..5650 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5680..5699 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5722..5741 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5785..5804 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5832..5851 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5881..5900 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5913..5932 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 5955..5974 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6001..6020 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6043..6062 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6091..6110 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6140..6159 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6182..6201 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6245..6264 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6292..6311 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6341..6360 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6373..6392 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6415..6434 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6461..6480 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6503..6522 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6551..6570 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6600..6619 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6642..6661 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6705..6724 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6752..6771 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6801..6820 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6833..6852 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6875..6894 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6921..6940 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 6963..6982 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 7011..7030 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 7060..7079 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 7102..7121 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
rep_origin 1
Label Gene Product Position Strand
p15A ori — — 8767..9312 Rev
DNA Sequence
TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTTGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGCGGAGCCTATGGAAA
terminator 2
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB — 7155..7227 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
T7Te terminator — — 7242..7270 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG