pEM414

248766 Synthetic Bacterial Expression

Plasmid Information

Purpose
20xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly
Depositor
Carolina Tropini
Insert
T7Te-rrnBT1-20xTetO
Available Since
Dec. 17, 2025
Availability
Available

Sequence Information

Length
3,411 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
KanR aph(3')-Ia aminoglycoside phosphotransferase 1150..1966 Rev
DNA Sequence
ATGAGCCATATTCAACGGGAAACGTCTTGCTCCAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATCGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
protein_bind 20
Label Gene Product Position Strand
tet operator tetO — 38..57 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 85..104 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 134..153 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 166..185 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 208..227 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 254..273 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 296..315 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 344..363 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 393..412 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 435..454 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 498..517 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 545..564 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 594..613 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 626..645 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 668..687 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 714..733 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 756..775 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 804..823 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 853..872 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 895..914 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
rep_origin 1
Label Gene Product Position Strand
p15A ori — — 2560..3105 Rev
DNA Sequence
TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTTGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGCGGAGCCTATGGAAA
terminator 2
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB — 948..1020 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
T7Te terminator — — 1035..1063 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG