UBC64

25143 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
ubc64.248.414 (UBE2Q1 Human)
Tags
His tag with thrombin cleavage
Mutation
contains UBC domain; residues 248-414
Available Since
Oct. 31, 2018
Availability
Available

Sequence Information

Length
908 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 59..77 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 86..104 Fwd
DNA Sequence
CTGGTTCCGCGTGGTAGT
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 634..652 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 718..766 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG