CBX3 (3DM1)

25242 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
CBX3_05 (CBX3 Human)
Tags
His
Available Since
Aug. 13, 2010
Availability
Available

Sequence Information

Length
894 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 36..54 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 66..87 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
6xHis — 6xHis affinity tag 290..308 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
rep_origin 1
Label Gene Product Position Strand
f1 ori — — 458..886 Fwd
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATT
terminator 1
Label Gene Product Position Strand
T7 terminator — — 374..422 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG