NMT1 (3JTK)

25277 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
NMT1:T108-Q496 (NMT1 Human)
Tags
His
Mutation
Contains amino acids T108-Q496
Available Since
Aug. 24, 2010
Availability
Available

Sequence Information

Length
910 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 466..484 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 496..517 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
promoter 1
Label Gene Product Position Strand
T7 promoter — — 376..395 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 395..420 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC