SGF29 (3MET)

25315 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
SGF29_6 (SGF29 Human)
Tags
His
Mutation
Contains amino acids 115-293
Available Since
Aug. 16, 2010
Availability
Available

Sequence Information

Length
933 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 4
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 44..62 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 74..95 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
Factor Xa site — Factor Xa recognition and cleavage site 575..587 Rev
DNA Sequence
ATTGAGGGGAGG
Protein Sequence
IEGR
6xHis — 6xHis affinity tag 661..679 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 745..793 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG