SGF29 (3MEW)

25320 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
SGF29_23 (SGF29 Human)
Tags
His
Mutation
Contains amino acids 129-291
Available Since
Aug. 17, 2010
Availability
Available

Sequence Information

Length
902 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 4
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 34..52 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 64..85 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
Factor Xa site — Factor Xa recognition and cleavage site 523..535 Rev
DNA Sequence
ATTGAGGGGAGG
Protein Sequence
IEGR
6xHis — 6xHis affinity tag 603..621 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 687..735 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG