HRMT1L3

25508 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
HRMT1L3 (PRMT3 Human)
Tags
His
Mutation
contains amino acids 149-463 only
Available Since
Oct. 24, 2011
Availability
Available

Sequence Information

Length
855 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 151..169 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 178..196 Fwd
DNA Sequence
CTGGTTCCGCGTGGTAGT
Protein Sequence
LVPRGS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 52..71 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 71..96 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC