PAPSS2

25539 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
PAPSS2 (PAPSS2 Human)
Tags
His
Mutation
Contains kinase domain (residues 21-218)
Available Since
Aug. 30, 2010
Availability
Available

Sequence Information

Length
845 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 469..487 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 496..514 Fwd
DNA Sequence
CTGGTTCCGCGTGGTAGT
Protein Sequence
LVPRGS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 370..389 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 389..414 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC