pMal672H

25817 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
Cynthia Wolberger
Article
Feeser et al J Mol Biol. 2008 Jul 11. 380(3):520-31.
Insert
Rap1(672-827) (RAP1 Budding Yeast)
Tags
His6 and MBP
Available Since
July 26, 2010
Availability
Available

Sequence Information

Length
865 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
Genenase(TM) I site — recognition and cleavage site for Genen… 26..44 Fwd
DNA Sequence
CCGGGTGCGGCACACTAC
Protein Sequence
PGAAHY
TEV site — tobacco etch virus (TEV) protease recog… 74..95 Fwd
DNA Sequence
GAAAATTTATATTTTCAAGGT
Protein Sequence
ENLYFQG
thrombin site — thrombin recognition and cleavage site 560..578 Fwd
DNA Sequence
TTAGTTCCCCGGGGTTCT
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 578..596 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
protein_bind 2
Label Gene Product Position Strand
attB1 mutant version of attB — 47..72 Fwd
DNA Sequence
ACAAGTTTGTACAAAAAAGCAGGCT
attB2 mutant version of attB — 599..624 Rev
DNA Sequence
ACCACTTTGTACAAGAAAGCTGGGT