| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 51..69 | Fwd | |
|
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
|
|||||
| thrombin site | — | thrombin recognition and cleavage site | 78..96 | Fwd | |
|
DNA Sequence
CTGGTGCCACGCGGTTCT
Protein Sequence
LVPRGS
|
|||||
| S-Tag | — | affinity and epitope tag derived from p… | 102..147 | Fwd | |
|
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
|
|||||
| Factor Xa site | — | Factor Xa recognition and cleavage site | 177..189 | Fwd | |
|
DNA Sequence
ATTGAGGGTCGC
Protein Sequence
IEGR
|
|||||
| ubiquitin | Homo sapiens UBC | human ubiquitin | 189..417 | Fwd | |
|
DNA Sequence
ATGCAGATCTTCGTCAAGACGTTAACCGGTAAAACCATAACTCTAGAAGTTGAATCTTCCGATACCATCGACAACGTTAAGTCGAAAATTCAAGACAAGGAAGGCATTCCACCTGATCAACAAAGATTGATCTTTGCCGGTAAGCAGCTCGAGGACGGTAGAACGCTGTCTGATTACAACATTCAGAAGGAGTCGACCTTACATCTTGTCTTAAGACTAAGAGGTGGT
Protein Sequence
MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
|
|||||
| 6xHis | — | 6xHis affinity tag | 498..516 | Fwd | |
|
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 terminator | — | — | 582..630 | Fwd | |
|
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
|
|||||