| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 33..51 | Rev | |
|
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
|
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| TEV site | — | tobacco etch virus (TEV) protease recog… | 387..408 | Rev | |
|
DNA Sequence
GAAAACCTGTATTTTCAGGGA
Protein Sequence
ENLYFQG
|
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| enterokinase site | — | enterokinase recognition and cleavage s… | 417..432 | Rev | |
|
DNA Sequence
GACGACGACGACAAG
Protein Sequence
DDDDK
|
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| S-Tag | — | affinity and epitope tag derived from p… | 447..492 | Rev | |
|
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
|
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| thrombin site | — | thrombin recognition and cleavage site | 498..516 | Rev | |
|
DNA Sequence
CTGGTGCCACGCGGTTCT
Protein Sequence
LVPRGS
|
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| 6xHis | — | 6xHis affinity tag | 525..543 | Rev | |
|
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
|
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| TrxA | trxA | E. coli thioredoxin | 564..891 | Rev | |
|
DNA Sequence
ATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCC
Protein Sequence
MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA
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