| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 29..47 | Rev | |
|
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
|
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| TEV site | — | tobacco etch virus (TEV) protease recog… | 353..374 | Rev | |
|
DNA Sequence
GAAAACCTGTATTTTCAGGGA
Protein Sequence
ENLYFQG
|
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| enterokinase site | — | enterokinase recognition and cleavage s… | 383..398 | Rev | |
|
DNA Sequence
GACGACGACGACAAG
Protein Sequence
DDDDK
|
|||||
| S-Tag | — | affinity and epitope tag derived from p… | 413..458 | Rev | |
|
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
|
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| thrombin site | — | thrombin recognition and cleavage site | 464..482 | Rev | |
|
DNA Sequence
CTGGTGCCACGCGGTTCT
Protein Sequence
LVPRGS
|
|||||
| 6xHis | — | 6xHis affinity tag | 491..509 | Rev | |
|
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
|
|||||
| TrxA | trxA | E. coli thioredoxin | 530..857 | Rev | |
|
DNA Sequence
ATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCC
Protein Sequence
MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 901..926 | Fwd | |
|
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC
|
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