GRM7

28110 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
GRM7:DCC014-D04:C211307 (GRM7 Human)
Tags
His tag with TEV site
Mutation
contains ligand binding domain, residues 37-513.
Available Since
March 18, 2011
Availability
Available

Sequence Information

Length
856 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 161..179 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 200..221 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG
sig_peptide 1
Label Gene Product Position Strand
melittin signal sequence — signal sequence from honeybee melittin 86..149 Fwd
DNA Sequence
ATGAAATTCTTAGTCAACGTTGCCCTTGTTTTTATGGTCGTATACATTTCTTACATCTATGCG