USP21 (3MTN)

28112 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
USP21 (USP21 Human)
Tags
His tag with Thrombin cleavage
Mutation
catalytic domain only (amino acid residues 209-56…
Available Since
Feb. 28, 2011
Availability
Available

Sequence Information

Length
860 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 44..62 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 71..89 Fwd
DNA Sequence
CTGGTTCCGCGTGGTAGT
Protein Sequence
LVPRGS