DPP7 (3N0T)

28114 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
DPP7:SDC134-D09:C41107 (DPP7 Human)
Tags
His
Mutation
contains amino acid residues 28-492
Available Since
March 18, 2011
Availability
Available

Sequence Information

Length
838 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 156..174 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 195..216 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG
sig_peptide 1
Label Gene Product Position Strand
melittin signal sequence — signal sequence from honeybee melittin 81..144 Fwd
DNA Sequence
ATGAAATTCTTAGTCAACGTTGCCCTTGTTTTTATGGTCGTATACATTTCTTACATCTATGCG