UBH14

28158 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
E3 ligase UHRF2 (UHRF2 Human)
Tags
His tag with thrombin cleavage
Mutation
SRA Domain (residues 419-648)
Available Since
Feb. 28, 2011
Availability
Available

Sequence Information

Length
773 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 49..67 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 76..94 Fwd
DNA Sequence
CTGGTTCCGCGTGGTAGT
Protein Sequence
LVPRGS