EPHB3

28165 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
EPHB3 (EPHB3 Human)
Tags
His tag with TEV cleavage and Honeybee melittin S…
Mutation
Ligand binding domain (residues 39-211).
Available Since
March 18, 2011
Availability
Available

Sequence Information

Length
825 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 155..173 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 194..215 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG
sig_peptide 1
Label Gene Product Position Strand
melittin signal sequence — signal sequence from honeybee melittin 80..143 Fwd
DNA Sequence
ATGAAATTCTTAGTCAACGTTGCCCTTGTTTTTATGGTCGTATACATTTCTTACATCTATGCG