pET-sCS4-2T

28250 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
Yawen Bai
Article
Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.
Insert
single chain Cse4-Scm3-H4 with 2 Thrombin site
Tags
His
Mutation
A thrombin cutting site between Cse4 and Scm3. An…
Available Since
July 12, 2011
Availability
Available

Sequence Information

Length
910 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 4
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 35..53 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 293..311 Fwd
DNA Sequence
CTGGTTCCGCGTGGATCC
Protein Sequence
LVPRGS
thrombin site — thrombin recognition and cleavage site 542..560 Fwd
DNA Sequence
CTGGTTCCGCGTGGATCC
Protein Sequence
LVPRGS
8xHis — 8xHis affinity tag 746..770 Fwd
DNA Sequence
CACCACCACCACCACCACCACCAC
Protein Sequence
HHHHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 846..894 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG