| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 35..53 | Fwd | |
|
DNA Sequence
CATCATCACCACCATCAC
Protein Sequence
HHHHHH
|
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| thrombin site | — | thrombin recognition and cleavage site | 215..233 | Fwd | |
|
DNA Sequence
CTGGTTCCGCGTGGATCC
Protein Sequence
LVPRGS
|
|||||
| 8xHis | — | 8xHis affinity tag | 419..443 | Fwd | |
|
DNA Sequence
CACCACCACCACCACCACCACCAC
Protein Sequence
HHHHHHHH
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 terminator | — | — | 519..567 | Fwd | |
|
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
|
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