pQTEV-DGKA

31294 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Konrad Buessow
Article
Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:
Insert
diacylglycerol kinase, alpha (DGKA Human)
Tags
His and TEV
Available Since
March 25, 2013
Availability
Available

Sequence Information

Length
840 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
7xHis — 6xHis affinity tag 154..175 Fwd
DNA Sequence
CATCACCATCACCATCACCAT
Protein Sequence
HHHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 199..220 Fwd
DNA Sequence
GAGAATCTTTATTTTCAGGGA
Protein Sequence
ENLYFQG
promoter 1
Label Gene Product Position Strand
T5 promoter — — 43..88 Fwd
DNA Sequence
TCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATA
protein_bind 2
Label Gene Product Position Strand
lac operator — — 63..80 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
lac operator — — 95..112 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
terminator 1
Label Gene Product Position Strand
lambda t0 terminator — — 619..714 Fwd
DNA Sequence
GACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAAT