DNMT3B (3QKJ)

32044 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
DNMT3B (DNMT3B Human)
Tags
His (with TEV cleavage)
Mutation
PWWP domain only (amino acids 293-442)
Available Since
Feb. 22, 2012
Availability
Available

Sequence Information

Length
399 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 42..60 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 72..93 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG