| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 147..165 | Fwd | |
|
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
|
|||||
| thrombin site | — | thrombin recognition and cleavage site | 174..192 | Fwd | |
|
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
|
|||||
| T7 tag (gene 10 leader) | — | leader peptide from bacteriophage T7 ge… | 195..228 | Fwd | |
|
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 promoter | — | — | 48..67 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 67..92 | Fwd | |
|
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
|
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